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Proteomic Profiling of Bladders from Mice Exposed with Sodium Arsenite
Citation:
WINNIK, W. M., J. CHILAKAPATI, K. WALLACE, K. T. KITCHIN, AND P. A. ORTIZ. Proteomic Profiling of Bladders from Mice Exposed with Sodium Arsenite. Presented at Environmental Mutagen Society 39th Annual Meeting, San Juan, PUERTO RICO, October 18 - 22, 2008.
Impact/Purpose:
Chronic exposure to arsenic has been linked with human cancers of the bladder, kidney, lung, liver, and skin. However, the mechanism of arsenic mediated carcinogenesis is still unknown. We have performed a global proteomic analysis of the mice bladder which may produce information crucial to the understanding of its mode or modes of action.
Description:
Arsenic, an environmental contaminant, has been linked with cancer of the bladder in humans. To study the mode of action of arsenic, female CH3 mice were exposed to 85 ppm sodium arsenite in their drinking water for 30 days. Following the exposure a comparative proteomic analysis of the mice bladders was performed by a 2D difference gel electrophoresis (DIGE) approach. Differentially expressed proteins were identified by nanospray-liquid chromatography-tandem mass spectrometry (LC-MSMS). Forty-five protein spots that showed changes in expression have been identified; some of the pathways affected by these proteins are valine, leucine and isoleucine degradation, glycolysis, actin cytoskeleton signaling, and the metabolism of fatty acids, glutathione, and pyruvate. Protein expression changes resulting from the arsenic exposure may provide essential information for the elucidation of its mode of action.