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The Enterococci qPCR Method for Recreational Water Quality Testing: Background and Single Lab Performance
Citation:
HAUGLAND, R. A. The Enterococci qPCR Method for Recreational Water Quality Testing: Background and Single Lab Performance. Presented at Scientific Meeting on Development, Validation and Implementation of qPCR and PCR Methods for Use in Recreational Waters, Cincinnati, OH, May 13, 2008.
Impact/Purpose:
Genetic markers from the 16S ribosomal RNA gene for six species of bacteroides that have been identified from existing nucleotide databases as being common in humans and rarely found in other animals have been identified and incorporated into TaqMan qPCR assays. A similar marker for another species of bacteroides that has been previously reported as human-specific has also been incorporated into a TaqMan qPCR assay. These assays together with two previously published TaqMan qPCR assays for reportedly human-specific bacteroides groups have been evaluated for acceptable analytical sensitivity and range of quantification for their respective target sequences. We are also assisting NRMRL in the evaluation of TaqMan qPCR assays for two potentially human specific genetic markers that were isolated by the genomic fragment enrichment (GFE) technique. TaqMan qPCR assays have also been developed for E. coli and a group of Clostridium species including C. perfringens. These latter assays have been used to determine levels of their respective target sequences in the 2007 NEEAR study samples.
Description:
Slide Presentation given at the Scientific Meeting on Development, Validation and Implementation of qPCR and PCR Methods for Use in Recreational Waters