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Selecting for resistance to the Cry3Bb1 protein in a genetically diverse population of non-diapausing Western Corn Rootworm
Citation:
French, B., M. BAGLEY, C. Nielson, AND U. STOLZ. Selecting for resistance to the Cry3Bb1 protein in a genetically diverse population of non-diapausing Western Corn Rootworm. IN: Resistant Pest Management Newsletter, Michigan State University, East Lansing, MI, 14(2):54, (2008).
Impact/Purpose:
This research effort is designed to provide the risk assessment community with modern genetic tools for evaluating long-term risks of genetically modified (GM) crops. Molecular population genetic data can potentially reveal information about long-term trends in both pest populations targeted by plant-incorporated pesticides (PIPs) and non-target populations. EPA regulations require specific pest management strategies to slow the evolution of resistance in target pest populations in order to prolong the environmental benefits of GM crops (i.e., reduced use of chemical pesticides). A key component of this research effort will be to provide detailed information on pest biology, including gene flow and mating patterns in the wild (within species and between closely related species), geographic and chromosomal distribution of resistance alleles, and the additive and non-additive effects of genetic variation on resistance under selective pressures in the field. These data will be used to improve and validate models of insect resistance development with the goal of implementing best agricultural practices for delaying or preventing the development of pest resistance. These methods will be evaluated as a necessary component in the process of developing novel PIPs. Monitoring resistance in the field is also a key component of Bt-crop stewardship mandated by the EPA. We will develop potential resistance markers using candidate resistance genes. Potential resistance markers will be used to develop a rapid molecular screening tool for field analysis of resistance in the field. Molecular monitoring would be preferable over regular field monitoring of crop damage due to resistant insects since molecular monitoring can detect resistance before economic damage and before resistance is wide spread. Molecular monitoring can potentially be an early indicatory of resistance development and allow time for remediation efforts.
Description:
Abstract published in Resistant Pest Management Newsletter, a biannual newsletter of the Center for Integrated Plant Systems (CIPS) in cooperation with the Insecticide Resistance Action Committee (IRAC) and the Western Regional Coordinating Committee (WRCC-60).