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Development of a Microarray Detection Method for Waterborne Pathogens - MCEARD/BARB
Citation:
BRINKMAN, N., E. VILLEGAS, R. Francisco, F. W. SCHAEFER, T. NICHOLS, D. Roberts, AND P. Schaudies. Development of a Microarray Detection Method for Waterborne Pathogens - MCEARD/BARB. Presented at Joint EPA and DHS Conference on Real-World Applications and Solutions for Microbial Risk Assessment, Bethesda, MD, April 08 - 10, 2008.
Impact/Purpose:
Overarching Objectives and Links to Multi-Year Planning This task directly supports the 2003 Drinking Water Research Program Multi-Year Plan's long term goal 2 to "develop new data, innovative tools and improved technologies to support decision making by the Office of Water on the Contaminant Candidate List and other regulatory issues” under GRPA Goal 2 (Clean and Safe Water). The overarching objective is to provide Agency scientists, risk assessors, and regulators as well as to individuals outside of the Agency that work in the field of drinking water quality the methods they need to measure the occurrence of waterborne viral pathogens. The method improvements will facilitate the development of risk-based assessments and tools used by the Agency to set regulations, policies and priorities for protecting human health. Specific Objectives Subtask A: Improving sample collecting, virus concentration and sample preparation · Develop a less expensive alternative to the Virosorb 1 MDS filter. This work supports the UCMR and other monitoring activities (to be completed by 9/08 in support of LTG2) Subtask B: Molecular and Cultural Assays · To improve the reverse transcription step in reverse transcription-polymerase chain reaction (RT-PCR) assays. · Development of an integrated cell culture/RT-PCR assay that can be used for screening environmental samples. · Begin the development of a reporter gene cell culture system. · Use improved cell culture lines to develop assays for nonculturable or poorly growing viruses. · To use current molecular and cultural virus detection methods in a pilot study to quantitate the impact of disinfection on virus viability and occurrence. These projects will support the UCMR and the CCL2 and CCL3 and are expected to be completed by 9/08.
Description:
Presentation at the Joint EPA and DHS Conference on Real-World Applications and Solutions for Microbial Risk Assessment in Bethesda, MD, April 8-10, 2008