Description:

The objectives of this research are to improve on the current analytical methods for quantitative detection of infective coxsackie and echo viruses in drinking water. The specific objectives of this research are to: (1) Improve the sensitivity and specificity of IMS-PCR for infective coxsackie and echo virus detection in water samples with the use of monoclonal antibodies; (2) Improve the efficiency and quantitation capabilities of IMS-PCR with the use of molecular beacons; and (3) Determine and quantify the presence and viability of coxsackie and echo viruses in concentrated drinking water samples.

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2001 Progress Report

2000 Progress Report

Record Details:

Record Type:PROJECT( ABSTRACT )

Start Date:02/01/2000

Completion Date:02/01/2002

Record ID: 18659

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Related Organizations:

Role :OWNER

Organization Name :UNIVERSITY OF CALIFORNIA - RIVERSIDE

Mailing Address :900 University Ave

Citation :Riverside

State :CA

Zip Code :92521

Project Information:

Approach :

We propose to combine IMS-PCR with molecular beacons to increase the sensitivity and specificity of analysis of environmental water samples for coxsackie and echo viruses. The research approach will include the: 1) optimization of IMS-PCR with monoclonal antibodies to the coxsackie and echo viruses; 2) development of fluorogenic probes for the target viruses; 3) analysis of virus-seeded water with the IMS - molecular beacon combination, and 4) testing of protocols on seeded drinking water concentrates to enable an assessment of the sensitivity of the method to inhibitors present in environmental waters. The specificity and quantitative capability of the protocols will be assessed by comparing results to quantitative cell culture analysis. All experiments will also be conducted with poliovirus to provide a basis for comparison.

Cost :$321,784.00

Research Component :Drinking Water

Risk Paradigm :EXPOSURE

Approach :

We propose to combine IMS-PCR with molecular beacons to increase the sensitivity and specificity of analysis of environmental water samples for coxsackie and echo viruses. The research approach will include the: 1) optimization of IMS-PCR with monoclonal antibodies to the coxsackie and echo viruses; 2) development of fluorogenic probes for the target viruses; 3) analysis of virus-seeded water with the IMS - molecular beacon combination, and 4) testing of protocols on seeded drinking water concentrates to enable an assessment of the sensitivity of the method to inhibitors present in environmental waters. The specificity and quantitative capability of the protocols will be assessed by comparing results to quantitative cell culture analysis. All experiments will also be conducted with poliovirus to provide a basis for comparison.

Cost :$321,784.00

Research Component :M/DBP (MICROBIAL)

Risk Paradigm :RISK MANAGEMENT

Approach :

We propose to combine IMS-PCR with molecular beacons to increase the sensitivity and specificity of analysis of environmental water samples for coxsackie and echo viruses. The research approach will include the: 1) optimization of IMS-PCR with monoclonal antibodies to the coxsackie and echo viruses; 2) development of fluorogenic probes for the target viruses; 3) analysis of virus-seeded water with the IMS - molecular beacon combination, and 4) testing of protocols on seeded drinking water concentrates to enable an assessment of the sensitivity of the method to inhibitors present in environmental waters. The specificity and quantitative capability of the protocols will be assessed by comparing results to quantitative cell culture analysis. All experiments will also be conducted with poliovirus to provide a basis for comparison.

Cost :$321,784.00

Research Component :ARSENIC

Risk Paradigm :RISK MANAGEMENT

Approach :

We propose to combine IMS-PCR with molecular beacons to increase the sensitivity and specificity of analysis of environmental water samples for coxsackie and echo viruses. The research approach will include the: 1) optimization of IMS-PCR with monoclonal antibodies to the coxsackie and echo viruses; 2) development of fluorogenic probes for the target viruses; 3) analysis of virus-seeded water with the IMS - molecular beacon combination, and 4) testing of protocols on seeded drinking water concentrates to enable an assessment of the sensitivity of the method to inhibitors present in environmental waters. The specificity and quantitative capability of the protocols will be assessed by comparing results to quantitative cell culture analysis. All experiments will also be conducted with poliovirus to provide a basis for comparison.

Cost :$321,784.00

Research Component :Drinking Water

Risk Paradigm :RISK MANAGEMENT

Approach :

We propose to combine IMS-PCR with molecular beacons to increase the sensitivity and specificity of analysis of environmental water samples for coxsackie and echo viruses. The research approach will include the: 1) optimization of IMS-PCR with monoclonal antibodies to the coxsackie and echo viruses; 2) development of fluorogenic probes for the target viruses; 3) analysis of virus-seeded water with the IMS - molecular beacon combination, and 4) testing of protocols on seeded drinking water concentrates to enable an assessment of the sensitivity of the method to inhibitors present in environmental waters. The specificity and quantitative capability of the protocols will be assessed by comparing results to quantitative cell culture analysis. All experiments will also be conducted with poliovirus to provide a basis for comparison.

Cost :$321,784.00

Research Component :M/DBP (MICROBIAL)

Risk Paradigm :EXPOSURE

Approach :

We propose to combine IMS-PCR with molecular beacons to increase the sensitivity and specificity of analysis of environmental water samples for coxsackie and echo viruses. The research approach will include the: 1) optimization of IMS-PCR with monoclonal antibodies to the coxsackie and echo viruses; 2) development of fluorogenic probes for the target viruses; 3) analysis of virus-seeded water with the IMS - molecular beacon combination, and 4) testing of protocols on seeded drinking water concentrates to enable an assessment of the sensitivity of the method to inhibitors present in environmental waters. The specificity and quantitative capability of the protocols will be assessed by comparing results to quantitative cell culture analysis. All experiments will also be conducted with poliovirus to provide a basis for comparison.

Cost :$321,784.00

Research Component :ARSENIC

Risk Paradigm :EXPOSURE

Project IDs:

ID Code :R828040

Project type :EPA Grant