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A PBPK MODEL FOR EVALUATING THE IMPACT OF ALDEHYDE DEHYDROGENASE POLYMORPHISMS ON COMPARATIVE RAT AND HUMAN NASAL TISSUE ACETALDEHYDE DOSIMETRY
Citation:
TEEGUARDEN, J. G., M. S. BOGDANFFY, T. R. COVINGTON, Y. M. TAN, AND A. M. JARABEK. A PBPK MODEL FOR EVALUATING THE IMPACT OF ALDEHYDE DEHYDROGENASE POLYMORPHISMS ON COMPARATIVE RAT AND HUMAN NASAL TISSUE ACETALDEHYDE DOSIMETRY. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, 20(4):375-90, (2008).
Impact/Purpose:
The rat and human acetaldehyde PBPK models developed here can also be used as a bridge between acetaldehyde dose-response and mode of action data and similar data bases for other acetaldehyde producing nasal toxicants.
Description:
ABSTRACT: Acetaldehyde is an important intermediate in chemical synthesis and a byproduct of normal oxidative metabolism of several industrially important compounds including ethanol, ethyl acetate and vinyl acetate. Chronic inhalation of acetaldehyde leads to degeneration of the olfactory and respiratory epithelium in rats at concentrations >50 ppm and respiratory and olfactory nasal tumors at concentrations ≥750 ppm, the lowest concentration tested. Differences in the anatomy and biochemistry of the rodent and human nose, including polymorphisms in human high affinity acetaldehyde dehydrogenase (ALDH2) are important considerations for interspecies extrapolations the risk assessment for acetaldehyde. A physiologically-based pharmacokinetic model of rat and human nasal tissues was constructed for acetaldehyde to support a dosimetry-based risk assessment for acetaldehyde (Dorman et al, this issue). The rodent model was developed using published metabolic constants and calibrated using upper respiratory tract acetaldehyde extraction data. The human model reflects previously published tissue volumes, blood flows and acetaldehyde metabolic constants. ALDH2 polymorphism was represented in the human model as reduced rates of acetaldehyde metabolism. Steady state dorsal olfactory epithelial tissue acetaldehyde concentrations in the rat were predicted to be 409, 6287 and 12634 μM at non cytotoxic (50 ppm), and cytotoxic/tumorigenic exposure concentrations (750 and 1500 ppm), respectively. The human equivalent concentration to the rat NOAEL of 50 ppm based on steady state acetaldehyde concentrations from continual exposures was 67 ppm. Respiratory and Olfactory epithelial tissue acetaldehyde H+ (pH) were largely linear functions of exposure in rats and humans. The impact of presumed ALDH2 polymorphisms on human olfactory tissue concentrations was negligible; the high affinity, low capacity ALDH2 does not contribute significantly to acetaldehyde metabolism in the nasal tissues. The human equivalent acetaldehyde concentration for homozygous low activity was 66 ppm, 1.5 % lower than for the homozygous full activity phenotype. The rat and human acetaldehyde PBPK models developed here can also be used as a bridge between acetaldehyde dose-response and mode of action data and similar data bases for other acetaldehyde producing nasal toxicants.
