You are here:
COMPLEMENTARY MOLECULAR AND ELEMENTAL DETECTION OF SPECIATED THIOARSENICALS USING ESI-MS IN COMBINATION WITH A XENON-BASED COLLISION-CELL ICP-MS WITH APPLICATION TO FORTIFIED NIST FREEZE-DRIED URINE
Citation:
ELLIS, J., C. GALLAWA, J. CARUSO, JOHN T. CREED, S. CONKLIN, P. A. CREED, AND A. R. YOUNG. COMPLEMENTARY MOLECULAR AND ELEMENTAL DETECTION OF SPECIATED THIOARSENICALS USING ESI-MS IN COMBINATION WITH A XENON-BASED COLLISION-CELL ICP-MS WITH APPLICATION TO FORTIFIED NIST FREEZE-DRIED URINE. Analytical and Bioanalytical Chemistry. Springer, New York, NY, 390(7):1731-1737, (2008).
Impact/Purpose:
Develop analytical approaches to quantify reactive intermediates within the metabolic pathway that biotransforms inorganic arsenic to DMA(V). The ability to quantify these species will enhance the mode of action (within NHEERL) and the bioavailability / bioaccessibility research (within NERL).
Description:
The simultaneous detection of arsenic and sulfur in thio-arsenicals was achieved using xenonbased collision cell ICP-MS in combination with HPLC. In an attempt to minimize the 16O16O+ interference at m/z 32, both sample introduction and collision cell experimental parameters were optimized. Low flow rates (0.25mL/min) and a high methanol concentration (8%) in the mobile phase produced a four fold decrease in the m/z 32 background. A plasma sampling depth change from 3mm to 7mm produced a two fold decrease in background at m/z 32, with a corresponding
four fold increase in the signal associated with a high ionization surrogate for sulfur. The quadrupole and octopole bias were used as a kinetic energy discriminator between background and analyte ions, but a variety of tuning conditions produced similar (