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LITERATURE REVIEW OF MOLECULAR METHODS FOR SIMULTANEOUS DETECTION OF PATHOGENS IN WATER
Citation:
SCHAUDIES, P. AND D. A. ROBINSON. LITERATURE REVIEW OF MOLECULAR METHODS FOR SIMULTANEOUS DETECTION OF PATHOGENS IN WATER. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-07/128 (NTIS PB2008-108849), 2007.
Impact/Purpose:
to inform the public
Description:
This literature search is a review of molecular technologies (qPCR, microarray, microfluidics and lab-on-a-chip) for simultaneous detection of multiple waterborne pathogens in order to understand the state of the technology. The search content focuses on: pathogen detection without DNA or RNA amplification; pathogen detection with DNA or RNA amplification; sample concentration methodologies; whole methods (concentration and detection without DNA or RNA amplification); whole methods (concentration and detection with DNA or RNA amplification); molecular methods that differentiate viable versus non-viable microorganisms. While the primary focus is on waterborne pathogens in potable water, the literature search includes wastewater and emerging clinical methodologies as these may be transferable to potable water applications and reports on recent technological developments covering advances made in the last ten years.