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TOXICOPROTEOMICS AND ITS APPLICATION TO HUMAN HEALTH RISK ASSESSMENT
Citation:
GE, Y., M. E. BRUNO, P. A. ORTIZ, AND W. M. WINNIK. TOXICOPROTEOMICS AND ITS APPLICATION TO HUMAN HEALTH RISK ASSESSMENT. Presented at Human Proteome Organization (HUPO), 6th Annual World Congress, Seoul, SOUTH KOREA, October 06 - 10, 2007.
Impact/Purpose:
Research presentation.
Description:
Humans are exposed to a variety of environmental toxicants, and this together with a large number of interacting factors can contribute to an individual's risk for health. To understand the toxic mechanisms and/or modes of action for human health risk assessment, molecular characterization of the proteome is required for a complete understanding of chemical-induced toxic responses. To apply quantitative toxicoproteomic analysis to the identification of toxic mechanisms and modes of action as well as to biomarkers of exposure, toxicity, and effect for human health risk assessment, we have developed an integrated toxicoproteomic platform. This platform has been applied to the analysis of the effects of several environmental carcinogens such as arsenic, bromate, benzo[a]pyrene, and conazoles on protein expression, phosphorylation, and oxidation in mouse and rat liver, lung, thyroid, and kidney and in human cells. Key findings obtained from these studies including arsenic-induced alterations in protein expression and phosphorylation, and conazole-induced alteration in protein oxidation will be presented. In addition, several fundamental questions regarding risk assessment-oriented toxicoproteomic research for an effective and comprehensive understanding of modes of action and mechanisms of chemical induced-toxic responses and disease processes will also be discussed in this presentation