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Speciation analysis of arsenic in biological matrices by automated hydride generation-cryotrapping-atomic absorption spectrometry with multiple microflame quartz tube atomizer (multiatomizer).
Citation:
HERNANDEZ-ZAVALA, A., T. MATOUSEK, Z. DROBNA, D. S. Paul, F. WALTON, B. ADAIR, J. DEDINA, D. J. THOMAS, AND M. STYBLO. Speciation analysis of arsenic in biological matrices by automated hydride generation-cryotrapping-atomic absorption spectrometry with multiple microflame quartz tube atomizer (multiatomizer). JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. Royal Society of Chemistry, Cambridge, Uk, 23(3):342-351, (2008).
Impact/Purpose:
This paper describes an automated system for the oxidation state specific speciation of inorganic and methylated arsenicals by selective hydride generation - cryotrapping- gas chromatography - atomic absorption spectrometry with the multiatomizer.
Description:
This paper describes an automated system for the oxidation state specific speciation of inorganic and methylated arsenicals by selective hydride generation - cryotrapping- gas chromatography - atomic absorption spectrometry with the multiatomizer. The corresponding arsines are generated either exclusively from trivalent or from both tri- and pentavalent inorganic and methylated arsenicals depending on the presence of L-cysteine as a prereductant and/or reaction modifier. A TRIS buffer reaction medium is proposed to avoid narrow optimum concentration range observed for the L-cysteine modified reaction in HCl medium. Arsines are preconcentrated and separated in a Chromosorb filled U-tube. An automated cryotrapping unit, employing nitrogen gas formed upon heating in the detection phase for the displacement of the cooling liquid nitrogen, has been developed. The automation improves performance and increases throughput of the procedure.
