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COMPARISON OF MOLD CONCENTRATIONS IN INDOOR AND OUTDOOR AIR SAMPLED SIMULTANEOUSLY AND THEN QUANTIFIED BY MSQPCR
Citation:
MEKLIN, T., T. REPONEN, C. MCKINSTRY, S. CHO, S. A. GRINSHPUN, A. NEVALAINEN, A. VEPSALAINEN, R. A. HAUGLAND, G. LEMASTERS, AND S. J. VESPER. COMPARISON OF MOLD CONCENTRATIONS IN INDOOR AND OUTDOOR AIR SAMPLED SIMULTANEOUSLY AND THEN QUANTIFIED BY MSQPCR. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier Science Ltd, New York, NY, 382(1):130-134, (2007).
Impact/Purpose:
1. Develop and publish a standard method or guidance document for QPCR analysis of microorganisms in environmental samples (air and water filtrates and dust).a standard method or guidance document for QPCR analysis of microorganisms in environmental samples (air and water filtrates and dust). Publication will involve a consensus standards organization. 2. Use QPCR methods to monitor childhood exposures to mold as a part of field studies, in order to establish whether a relationship exists between molds encountered in indoor environments and asthma-related health problems.
Description:
Mold specific quantitative PCR (MSQPCR) was used to measure the concentrations of the 36 mold species in indoor and outdoor air samples that were taken simultaneously for 48 hours in and around 17 homes in Cincinnati, Ohio. The total spore concentrations of 353 per m3 of indoor air and 827 per m3 of outdoor air samples were significantly different (p < 0.05). However, only the concentrations of Aspergillus penicillioides, Cladosporium cladosporioides types 1 and 2 and C. herbarum were correlated in indoor and outdoor air samples (p value < 0.05 and sufficient data for estimate and absolute value rho estimate > 0.5). These results suggest that interpretation of the meaning of short-term (< 48 h) mold measurements in indoor and outdoor air samples must be made with caution.