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TURKEY FECAL MICROBIAL COMMUNITY STRUCTURE AND ECOLOGICAL FUNCTIONS REVEALED BY 16S RDNA AND METAGENOME SEQUENCES
Citation:
LU, J. AND J. W. SANTO-DOMINGO. TURKEY FECAL MICROBIAL COMMUNITY STRUCTURE AND ECOLOGICAL FUNCTIONS REVEALED BY 16S RDNA AND METAGENOME SEQUENCES . The Journal of Microbiology . Springer, New York, NY, 46(5):469-477, (2008).
Impact/Purpose:
SDWA
Description:
Turkey feces are an important source of fecal waste in the United States. With the exception of isolated studies on bacterial pathogens, little is known about the type of bacteria inhabiting the turkey gut. In order to understand the microbial diversity and functional genes associated with turkey fecal samples, 16S rDNA and metagenomic clone libraries were sequenced. The analysis of 382 16S rDNA sequences showed that the most abundant bacteria were closely related to Lactobacillales (47%), Bacillales (31%) and Clostridiales (11%). Actinomycetales and Enterobacteriales sequences were also identified but represented a smaller part of the community. Overall, these results suggest that the turkey fecal community structure is different from other domesticated animals. The analysis of 373 metagenomic sequences showed that most clones were similar to bacterial protein sequences (58%). Bacteriophage (10%) and avian viruses (3%) sequences were also represented in the metagenomic library. Most metagenomic sequences were similar to low G+C gram positive bacterial proteins, particularly from Lactobacillales (50%), Clostridiales (12%), and Bacillales (11%), which was in agreement with the predominant bacterial groups identified in the16S rDNA clone library. Some metagenomic sequences were similar to the genes encoding membrane proteins, lipoproteis and hydrolases previouosly found to show host-specificity to chicken and cow fecal metagenomes. Consequently, some of the isolated clones might be potential targets for the development fectal source tracking assays. Sequences similar to functional genes associated with the metabolism of nitrogen and sulfur containing compounds were also obtained.
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TURKEY FECAL MICROBIAL COMMUNITY STRUCTURE AND ECOLOGICAL FUNCTIONS REVEALED BY 16S RDNA AND METAGENOME SEQUENCES