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SAMPLE EXTRACTION AND GC-MS ANALYSIS FOR POLAR VOLATILE ORGANIC COMPOUNDS (PVOCS) IN LIQUID BIOLOGICAL MEDIA
Citation:
PLEIL, J. D., J. R. SOBUS, H. HUBBARD, AND M. C. MADDEN. SAMPLE EXTRACTION AND GC-MS ANALYSIS FOR POLAR VOLATILE ORGANIC COMPOUNDS (PVOCS) IN LIQUID BIOLOGICAL MEDIA. Presented at Environmental Analysis Conference 2007, Wellington, NEW ZEALAND, February 06 - 09, 2007.
Impact/Purpose:
Objectives for this task include the following:
o Investigate the current research on biomarkers of exposure for humans (ILSI documents).
o Develop better measurement methods and procedures for collecting biomarkers of exposure in human research studies.
o Conduct advanced statistical analyses on existing urinary biomarker data generated from EPA funded studies.
o Identify and provide valuable data inputs for modelers in their toxicokinetic and exposure models.
o Use modeling tools to understand the exposures of humans to chemicals related to biomarkers of exposure.
o Form a collaborative partnership with CDC to develop analytical methods for chemicals used as biomarkers of exposure in humans.
o Develop statistical methods and models to assess human exposures to chemicals of concern.
Description:
Current approaches for assessing the cumulative exposures and effects from broad classes of environmental stressors incorporate the measurement of specific groups of endogenous compounds in human biological fluids. Recent focus has been on interpreting patterns of differentially expressed proteins and large molecule adducts (e.g hemoglobin, albumin, DNA) which are difficult to measure. In contrast, this work exploits classes of simple polar volatile organic compounds (PVOCs) that are abundant and ubiquitous products of human metabolism and can be found in non-invasively collected breath or urine samples. Methodology for measuring metabolic PVOCs is presented using standard laboratory tools and modest gas chromatography/mass spectrometry equipment.