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SYSTEMIC BIOMARKERS AND CARDIAC GENE EXPRESSION PROFILES OF RAT DISEASE MODELS EMPLOYED IN AIR POLLUTION STUDIES
Citation:
RICHARDS, J. E., W. O. WARD, S. D. HESTER, M. SCHLADWEILER, A. D. LEDBETTER, R. H. JASKOT, A. NYSKA, R. R. GOTTIPOLU, G. WALLENBORN, J. SHANNAHAN, AND U. P. KODAVANTI. SYSTEMIC BIOMARKERS AND CARDIAC GENE EXPRESSION PROFILES OF RAT DISEASE MODELS EMPLOYED IN AIR POLLUTION STUDIES. Presented at American Thoracic Society Annual Meeting, San Francisco, CA, May 18 - 23, 2007.
Description:
Cardiovascular disease (CVD) models are used for identification of mechanisms of susceptibility to air pollution. We hypothesized that baseline systemic biomarkers and cardiac gene expression in CVD rat models will have influence on their ozone-induced lung inflammation. Male 12-14 wks old Wistar Kyoto (WKY, healthy), Spontaneously Hypertensive (SHR), Stroke-Prone SHR (SHRSP), Heart Failure SHR (SHHF) and Diabetic (JCR) rats were exposed to air or ozone (1 ppm) for 4 hrs. Serum cholesterols, coagulation parameters, and lung inflammation (via BAL) were examined. Cardiac gene expression was evaluated using Affymetrix arrays only in air-exposed rats (n=4/strain). JCR and SHHF with obese phenotype had high total cholesterol and HDL relative to other strains, but not LDL. This was associated with reduced expression of CD36, a fatty acid transporter. Blood coagulation parameters were comparable between WKY and JCR but increased in SHRSP and SHR. These phenotypes reflected in dysregulation of coagulation and lipid metabolism genes. PCA demonstrated remarkable strain-related differences in gene expression. Ozone-induced lung inflammation was in order of SHR=SHHF