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QUANTIFICATION OF FLUOROTELOMER-BASED CHEMICALS IN MAMMALIAN MATRICES BY MONITORING PERFLUOROALKYL CHAIN FRAGMENTS WITH GC/MS
Citation:
HENDERSON, W. M., E. J. WEBER, S. E. DUIRK, J. W. WASHINGTON, AND M. A. SMITH. QUANTIFICATION OF FLUOROTELOMER-BASED CHEMICALS IN MAMMALIAN MATRICES BY MONITORING PERFLUOROALKYL CHAIN FRAGMENTS WITH GC/MS. Journal of Chromatography B. Elsevier Science Ltd, New York, NY, 846(1-2):155-161, (2007).
Impact/Purpose:
The general goals of this research are to meet OPPT's need for data and information regarding the stability of TBPPs in soils. We plan to address these needs with a series of lab experiments in which FBPs are exposed to selected natural and amended soils. We anticipate that, as we learn about the general behavior of FBPs in soils, we will develop a protocol for testing FBPs in soils that has procedures similar to the guidelines described in OECD 307.
Description:
Perfluorocarboxylic acids (PFCAs), namely perfluorooctanoic acid (PFOA) and perfluorononanoic acid (PFNA), have been identified as persistent, bioaccurnulative and potentially toxic compounds. The structural analog, 8-2 fluorotelomer alcohol (8-2 FTOH) is considered the probable precursor of these stable metabolites. Because simultaneous quantification is needed for volatile and non-volatile perfluorinated chemicals (PFCs) in complex matrices, a GC/MS method was developed and tested based on selected ion monitoring of perfluorinated alkyl parent chain fragment ions. Although the method requires a derivatization step, combined GC/MS analysis of PFCA -me's and FTOHs increases analytical efficiency and decreases sample analysis time. The method instrument detection limits are between 7.1 and 24.5 ng/mL extract (MTBE), and the method quantification limits are below 50 ng/mL serum or ng/g liver for all PFCs investigated. Recoveries from mouse serum and liver homogenates, which were spiked with FTOHs and PFCAs at levels of 25 and 200 ng/mL or ng/g, ranged from 81 to 101%. Finally, the utility of the method was demonstrated by dosing male CD-I mice with 30 mg/kg-BW of 8-2 FTOH and quantifying PFCs 6 h post-treatment. The advantages of this method are (1) the simultaneous detection of both volatile and non-volatile fluorotelomer-based chemicals in complex matrices such as mammalian tissues, (2) as a confirmatory method to LC-MS/MS, and (3) as an alternative method of analysis for laboratories without access to LC-MS/MS.