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DELTAMETHRIN AND PERMETHRIN DECREASE SPONTANEOUS ACTIVITY IN NEURONAL NETWORKS IN VITRO.
Citation:
SHAFER, T. J., S. O. RIJAL, AND G. W. GROSS. DELTAMETHRIN AND PERMETHRIN DECREASE SPONTANEOUS ACTIVITY IN NEURONAL NETWORKS IN VITRO. Presented at 5th International Meeting on Substrate-integrate Microelectrode Arrays, Reutlingen, GERMANY, July 04 - 07, 2006.
Description:
Effects of pyrethroid insecticides on spontaneous electrical activity were investigated in primary cultures of cortical or spinal cord neurons grown on microelectrode arrays. Bicuculline (40 ¿M) was utilized to block fast GABAergic transmission, and concentration-dependent effects of deltamethrin (1 nM - 5 ¿M) and permethrin (10 nM - 50 ¿M) were examined. Both compounds caused a concentration-dependent reduction in spike frequency (#spikes/min), as well as the number of bursts/minute. Deltamethrin was more potent than permethrin, with respective IC50 values of ~200 nM and ~4 ¿M for spike frequency inhibition in both tissues, respectively. Onset of effects was rapid and reductions in spike rate could only be partially reversed with successive complete media replacements. These results demonstrate that both deltamethrin and permethrin are potent inhibitors of network activity in cortical and spinal cord cultures in vitro, and suggests that despite differences in the clinical signs of poisoning, similar modes of action at the network level may contribute to effects of these compounds.