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A FLUORESCENCE-BASED SCREENING ASSAY FOR DNA DAMAGE INDUCED BY GENOTOXIC INDUSTRIAL CHEMICALS
Citation:
KAILASAM, S. AND K. R. ROGERS. A FLUORESCENCE-BASED SCREENING ASSAY FOR DNA DAMAGE INDUCED BY GENOTOXIC INDUSTRIAL CHEMICALS. CHEMOSPHERE. Elsevier Science Ltd, New York, NY, 66(1):165-171, (2007).
Impact/Purpose:
The scientific modeling expertise of NERL/AMD technical staff often results in requests for staff to provide timely support and advice to EPA Management in the event of a national emergency and to perform the research on model development and evaluation in support of the development of early response models. Flow and dispersion in urban areas is extremely complex and not well characterized in spite of their importance to homeland security and emergency response applications. Specifically, understanding the transport and fate of pollutants in urban canopies is critical for implementing air quality standards, performing risk assessments, developing environmental management strategies, supporting human exposure and health effects studies. Recently, though, much focus has been placed on supporting the development of homeland security tools needed to prepare for and respond to malicious attacks with toxic chemicals. Toward this goal, laboratory studies have been conducted in EPA's Meteorological Wind Tunnel for idealized urban settings, for very complex settings such as lower Manhattan surrounding the World Trade Center (WTC) site, and for very building specific cases such as the Pentagon. Continued studies in a variety of urban morphologies and release scenarios are needed to support a more thorough understanding of urban dispersion and the development of refined numerical modeling approaches.
Description:
The possibility of deliberate or accidental release of toxic chemicals in industrial, commercial or residential settings has indicated a need for rapid, cost-effective and versatile monitoring methods to prevent exposures to humans and ecosystems. Because many toxic industrial chemicals are not only acutely toxic but genotoxic as well, there is a need for simple and rapid screening techniques to measure these genotoxins.
A rapid screening assay to detect chemically-induced DNA damage resulting from exposure of surrogate DNA to genotoxic compounds is reported.