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EFFECTS OF AMBIENT PM ON CYTOKINE PRODUCTION IN MOUSE MACHROPHAGES AND EPITHELIAL CELLS
Citation:
BOYLES, R., IAN I. GILMOUR, AND I. JASPERS. EFFECTS OF AMBIENT PM ON CYTOKINE PRODUCTION IN MOUSE MACHROPHAGES AND EPITHELIAL CELLS. Presented at Society of Toxicology Annual Meeting, San Deigo, CA, March 05 - 09, 2006.
Description:
The objective of the Multi-Site Ambient Particle Study (MAPS) was to collect ambient particles from different geographical regions, examine their health effects using various in vitro and in vivo experimental approaches, and ultimately relate the health effects to particle components and pollution sources. Size fractionated airborne particles were collected in six different locations in the United States and made available to investigators in several different laboratories. The objective of this study is to compare the effects of these ambient particles on cytokine production in different lung cell types using in vitro models of mouse macrophages and lung epithelial cells. Briefly, MH-S cells, a mouse lung macrophage cell line, and LA-4 cells, a mouse lung epithelial cell line, were exposed to 10 or 100¿g/ml of either the coarse or fine particle fraction and examined for IL-6 expression 24 hours post-exposure. Both coarse and fine particle fractions dose-dependently upregulated IL-6 production in MH-S cells and these responses varied moderately with geographical location. Interestingly, the coarse fraction induced significantly greater IL-6 production in MH-S cells than the fine fraction, independently of geographical location. In contrast, both fine and coarse particle fractions upregulated IL-6 production in LA-4 cells and the magnitude of these responses varied with collection site. Taken together, these results indicate that regardless of geographic location, coarse and fine particle fractions exert different effects in lung macrophages and epithelial cells, with epithelial cells being more susceptible to health effects induced by fine particle fractions. In addition, our preliminary analysis suggests that geographical collection site may not be a good predictor of health effects in different lung cell types. Data obtained from this study will help distinguish the role of different lung cell types in particle-induced health effects seen in vivo.