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COMPUTER-ASSISTED MOTION ANALYSIS OF SPERM FROM THE COMMON CARP
Citation:
Toth, G., S. Jackson, H. W. McCarthy, J. A. Torsella, AND M. Smith. COMPUTER-ASSISTED MOTION ANALYSIS OF SPERM FROM THE COMMON CARP. Journal of Fish Biology 47(6):986-1003, (1995).
Description:
Computer-assisted semen analysis (CASA) technology was applied to the measurement of sperm motility parameters in the common carp Cyprinus carpio. Activated sperm were videotaped at 200 frames s-1 and analysed with the CellTrak/S CASA research system. The percentage of motile cells and both sperm head curvilinear velocity and straight-line velocity were measured following exposure of carp sperm to three predilution conditions and activation in media of differing ionic strengths and osmotic pressures. The highest percentage of motile sperm was obtained following predilution of sperm in seminal plasma and activation in Na-HEPES buffer pH 8-0. This level of motility was equalled after predilution in 200 mm KC1 for 2h. Straight-line velocities and curvilinear velocities of 130 um s-1 and 210 um s-1, respectively, were observed. Duration of motility was higher under seminal plasma predilution conditions (over 50% motile sperm at 55 s post-activation). The application provides a sound conditions (over 50% motile sperm at 55 s post-activation). The application provides a sound basis for the assessment of sperm characteristics in fish.