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THE RELATIONSHIP BETWEEN OZONE-INDUCED LUNG INJURY, ANTIOXIDANT COMPENSATION AND UNDERLYING CARDIOVASCULAR DISEASE (CVD).
Citation:
KODAVANTI, U. P., M. SCHLADWEILER, A. D. LEDBETTER, K. M. CRISSMAN, G. WALLENBORN, J. E. RICHARDS, R. H. JASKOT, R. THOMAS, AND G. E. HATCH. THE RELATIONSHIP BETWEEN OZONE-INDUCED LUNG INJURY, ANTIOXIDANT COMPENSATION AND UNDERLYING CARDIOVASCULAR DISEASE (CVD). . Presented at American Thoracic Society Annual Meeting, San Diego, CA, May 19 - 24, 2006.
Description:
Increased levels of oxidants and compromised compensatory response are associated with CVD susceptibility. We hypothesized that rat strains demonstrating genetic CVD will have lower levels of antioxidants and greater ozone-induced pulmonary injury relative to healthy strains. Male 12-15 wk old Sprague Dawley (SD), Wistar (WIS), Wistar Kyoto (WKY), Spontaneously Hypertensive (SH), Stroke-prone SH (SHSP), Heart Failure Hypertensive (SHHF), Diabetic (JCR), and Fawn Hooded Hypertensive (FHH) rats were exposed nose-only to ozone, 0.0, 0.25, 0.5 or 1.0 ppm for 4 h. BAL fluid protein, neutrophils, ascorbate, total glutathione and antioxidant enzymes were measured at 0 or 20 h later. In general, rat strains with high concentration of glutathione in BALF also had high levels of ascorbate. Rat strains with CVD did not necessarily have lower antioxidants; JCR and SHHF rats had the highest glutathione and ascorbate in their BAL fluid. Ozone caused significant depletion in BALF ascorbate but not glutathione in a dose-dependent manner in all rat strains. SH rats were least affected but had lowest ascorbate in BAL fluid. Ozone also caused dose-dependent increases in BALF protein in all strains with SHSP, SHHF, SH, WIS having the greatest effect. Neutrophilic inflammation was greater in FHH, WIS, SHSP and WKY rats relative to other strains. Ozone also caused differential strain-dependent changes in breathing parameters. This study demonstrated that the relationship between antioxidants level, underlying CVD and ozone-induced inflammatory response may not be generalized for all rat strains; other phenotypic and genetic differences may play a role in determining susceptibility to ozone. (Does not reflect USEPA policy. This research was supported in part by UNC/EPA CT829471).