Office of Research and Development Publications

RECOVERY AND ASSAY OF VIRUSES FROM BIOSOLIDS

Citation:

FOUT, G. RECOVERY AND ASSAY OF VIRUSES FROM BIOSOLIDS. Presented at Pathogen Equivalency Committee Retreat, College Corner, OH, September 20 - 21, 2005.

Impact/Purpose:

The objectives of this task are to:

  • Optimize a method for detecting and determining the viability of Ascaris ova in a variety of sludge types
  • Develop a Standard Operating Procedure for Ascaris ova and perform a single laboratory validation
  • Optimize a method for detecting pathogenic viruses in a variety of sludge types
  • Develop a Standard Operating Procedure for viruses and perform a single laboratory validation
  • Conduct an exposure measurement workshop on pathogens in sludg
  • e

    Description:

    In a review of EPA's rule on sewage sludges, the National Research Council recommended that EPA develop improved methods for pathogens using modern technologies. They specifically mentioned viral pathogens that cannot be cultured and those that are difficult to culture. As a result of this recommendation, EPA funded work under Contract # 68-C-00159 with the University of Cincinnati to develop "Improved Methods for Virus Detection in Biosolids." This talk describes the areas and status of the research that is being conducted under the work assignment.

    Record Details:

    Record Type:DOCUMENT( PRESENTATION/ SLIDE)
    Product Published Date:09/20/2005
    Record Last Revised:06/21/2006
    Record ID: 141780