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DISEASE-SPECIFIC SUSCEPTIBILITY TO ACUTE OZONE-INDUCED INJURY AND INFLAMMATION IN EIGHT RAT STRAINS
Citation:
KODAVANTI, U. P., M. SCHLADWEILER, A. D. LEDBETTER, G. WALLENBORN, R. H. JASKOT, AND J. E. RICHARDS. DISEASE-SPECIFIC SUSCEPTIBILITY TO ACUTE OZONE-INDUCED INJURY AND INFLAMMATION IN EIGHT RAT STRAINS. Presented at 45th Annual Society of Toxicology Meeting 2006, San Diego, CA, March 05 - 09, 2006.
Description:
Susceptibility to environmental pollutant-induced injuries may be influenced by presence of disease and genetic make-up. To identify disease-specific susceptibility phenotype, we used eight rat strains with or without genetic cardiovascular disease. Male 12-15 wk old Sprague Dawley (SD), Wistar (WIS), Wistar Kyoto (WKY), Spontaneously Hypertensive (SH), Stroke-Prone SH (SHSP), Heart Failure Hypertensive (SHHF), Diabetic (JCR), and Fawn Hooded Hypertensive (FHH) rats were exposed nose-only to ozone, 0.0, 0.25, 0.5 or 1.0 ppm for 4 h. Lung injury and inflammation were evaluated at 0 or 20 h later by analysis of bronchoalveolar lavage fluid (BALF). There were strain-related differences in base-line levels of lung inflammation and protein (neutrophils: SH=SHSP>SD>WIS=SHHF=WKY>FHH>>JCR; protein: SHHF>SH>JCR>SHSP=WKY>FHH>WIS=SD) suggesting that rat strains with cardiovascular disease exhibit border-line pulmonary hypertension. In general, ozone effects at 0 h were less than those at 20 h. Ozone caused dose-dependent increases in BALF protein and albumin (1.0 ppm ozone-induced increase from base line; SHSP>SHHF=SH=WIS=WKY>FHH>SD>JCR), however, effect on LDH activity was minimal. N-acetylglucosaminidase activity was only slightly elevated at 1.0 ppm ozone in all but SD and SH rats. ?-Glutamyl transferase activity increased at 1.0 ppm only in FHH, SHRSP and SHHF rats. Ozone caused dose-dependent but variable degree of neutrophilic influx with only minimal effects on levels of alveolar macrophages in all rat strains. Increase in neutrophils from base line levels at 1.0 ppm ozone was in the order of FHH>WIS>SHSP>WKY=JCR>SHHF>SD>SH. The susceptibility to ozone differed between injury parameters in a given rat strain. Ozone-induced inflammatory and injury responses were also rat strain and disease-specific. (Abstract does not reflect USEPA policy. This research was supported in part by UNC/EPA CT829471).