Citation:

WALLENBORN, G., M. SCHLADWEILER, A. D. LEDBETTER, A. NYSKA, J. A. JOHNSON, R. THOMAS, R. H. JASKOT, J. E. RICHARDS, AND U. P. KODAVANTI. COMPARISON OF CARDIOPULMONARY RESPONSES OF WISTAR KYOTO (WKY) AND STROKE PRONE SPONTANEOUSLY HYPERTENSIVE RATS (SHRSP) TO PARTICULATE MATTER (PM) EXPOSURE. Presented at 45th Annual Society of Toxicology Meeting 2006, San Diego, CA, March 05 - 09, 2006.

Description:

Although a clear link between cardiopulmonary disease and an increased susceptibility to air pollution has been established epidemiologically, the mechanistic link remains undefined. Animal models of disease are widely used to investigate this link. Here we compare the cardiopulmonary response of SHRSP to healthy WKY rats to oil combustion PM exposure. Male rats (12-15 wks age) were intratracheally instilled with saline or 1.11, 3.33 or 8.33 mg/kg of PM. Responses were analyzed 4h or 24h later. Baseline levels of protein and albumin, but not lactate dehydrogenase (LDH) or N-acetyl glucosaminidase (NAG) activities in the bronchoalveolar lavage fluid (BALF) of control SHRSP were higher than control WKY. Additionally, effects of PM instillation on BALF levels of protein, albumin and -glutamyltransferase (GGT) activity were exacerbated in SHRSP as compared to WKY, suggesting more vascular leakage in SHRSP. Total numbers of lavageable cells and neutrophils increased in the BALF of both strains. Histopathology evaluation revealed treatment related lesions in the lung, but not heart, of both strains. Electron microscopy images showed no apparent acute exposure-related pathology to cardiac mitochondria in either strain. These data suggest SHRSP are more susceptible than WKY to lung injury following PM exposure. Furthermore, a single instillation of PM causes lung inflammation but no apparent cardiac pathology or mitochondrial structural damage in either strain. (Abstract does not represent USEPA policy. This research was supported in part by UNC/EPA CT829471.)

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