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SABRE MULTI-LAB, STATISTICALLY-BASED MICROCOSM STUDY FOR TCE SOURCE ZONE REMEDIATION (ABSTRACT ONLY)
Citation:
HARKNESS, M., A. FISHER, R. ROYER, A. POSSOLO, M. LEE, E. E. MACK, J. PAYNE, D. MAJOR, J. ROBERTS, S. DWORATZEK, C. M. ACHESON, R. F. HERRMANN, AND X. MAO. SABRE MULTI-LAB, STATISTICALLY-BASED MICROCOSM STUDY FOR TCE SOURCE ZONE REMEDIATION (ABSTRACT ONLY). In Proceedings, Fifth International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey, CA, May 22 - 25, 2006. Battelle Press, Columbus, OH, B-44, ISBN1574771574, (2006).
Impact/Purpose:
To inform the public.
Description:
SABRE (source area bioremediation) is a public/private consortium of twelve companies, two government agencies, and three research institutions whose charter is to determine if enhanced anaerobic bioremediation can result in effective and quantifiable treatment of chlorinated solvent DNAPL source areas. The focus of this 4-year, $5.7 million dollar research and development project is a field site in the United Kingdom containing a DNAPL source area with groundwater concentrations exceeding several hundred mg/L TCE. Prior to field implementation, a large-scale, multi-laboratory microcosm study is being performed to determine the optimal electron donor, supplemental nutrient, and bioaugmentation combination to support reductive dechlorination of TCE in site soil and groundwater. The study consists of 168 bottles (including unamended and sterile controls) distributed between four industrial laboratories (Dupont, GE, SiREM, and Terra Systems) and employs a statistically-based fractional factorial experimental design to test the impact of six electron donors (lactate, acetate, methanol, SRS (soybean oil), hexanol, butyl acetate), bioaugmentation with KB-1 bacterial culture, addition of supplemental nutrients (ammonia, phosphate, yeast extract), and two TCE levels (100 mg/L and 400 mg/L) on TCE dechlorination. Hexanol and butyl acetate are novel donors that have the potential to partition into the TCE DNAPL phase, creating a long-lived source of electron donor in the subsurface. The microcosm study began on February 17, 2005. After 120 days, 73 of the amended bottles have reached complete dechlorination of TCE to ethene. These bottles represent 51% of the amended bottles in the study. SRS, methanol, and lactate have been the most effective electron donors to date, although all of the donors tested have supported complete dechlorination in at least nine bottles. Bioaugmentation and the addition of supplemental nutrients also appear to be important in promoting complete dechlorination. Most of the amended bottles that have not yet reached complete dechlorination continue to progress in that direction. Total VOC mass balances incorporating TCE and daughter products in three phases (soil, water, headspace) within the microcosms have been excellent. The study has also displayed good reproducibility between participating laboratories. Statistical analysis of the data using ANOVA (analysis of variance) techniques has begun and will determine both main effects and two- or three-way interactions for all the experimental variables using multiple metrics to assess success. The data will be combined with microbial measurements (PLFA, DHC, DGGE) to develop a comprehensive kinetic model of the dechlorination system to be used to design follow-up column studies and the field pilot system.
URLs/Downloads:
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SABRE Multi-Lab Statistically Based Microcosm Study for TCE Source Zone Remediation (PDF, NA pp, 22 KB, about PDF)