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THE MUTATION SPECTRA OF DRINKING WATER SAMPLES USING THE BASE-SPECIFIC TA7000 STRAINS OF SALMONELLA IN THE MICROSUSPENSION ASSAY
Citation:
UMBUZEIRO, G., S. H. WARREN, AND L. D. CLAXTON. THE MUTATION SPECTRA OF DRINKING WATER SAMPLES USING THE BASE-SPECIFIC TA7000 STRAINS OF SALMONELLA IN THE MICROSUSPENSION ASSAY. MUTATION RESEARCH. Elsevier Science Ltd, New York, NY, 609(1):26-33, (2006).
Impact/Purpose:
To characterize water samples from two drinking water treatment plants in Sao Paulo, Brazil
Description:
Previous studies showed that disinfected drinking water samples gave mutagenic spectra typical of halogenated furanones. In this study, we used the TA7000 base-¿specific Salmonella typhimurium tester strains to characterize water samples from two drinking water treatment plants (DWTPs) in Sao Paulo, Brazil. Small sample sizes required the use of a microsuspension protocol. Therefore, the microsuspension criteria (e.g., spontaneous range of revertants) and protocol were developed and compared to normal protocols. Samples from the two DWTPs although very different in relation to the mutagenic activity of the natural pH extract detected with TA98/YG1041 gave similar responses in the TA7000 series, and caused primarily GC to TA transversions. It is likely that the halogenated disinfection byproducts, generated during the chlorination of water, are causing the observed effects of the waters analyzed in this study with the TA7000 strains.