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LIPID ANALYSIS TO DETERMINE THE EFFECT OF A SOURCE REMEDIAL TECHNOLOGY IN MICROBIAL ECOLOGY
Citation:
AZADPOUR-KEELEY, ANN. LIPID ANALYSIS TO DETERMINE THE EFFECT OF A SOURCE REMEDIAL TECHNOLOGY IN MICROBIAL ECOLOGY. In Proceedings, Eigth International In Situ and On-Site Bioremediation Symposium, Baltimore, MD, June 06 - 09, 2005. Battelle Press, Columbus, OH, D-28, ISBN1574771523, (2005).
Impact/Purpose:
To observe Microbial community structures and related changes in the subsurface environment following in situ chemical oxidation (ISCO) treatment at Launch Complex 34, Cape Canaveral Air Station, FL.
Description:
Microbial community structures and related changes in the subsurface environment were investigated following in situ chemical oxidation (ISCO) treatment at Launch Complex 34, Cape Canaveral Air Station, Florida. The site has dense non-aqueous phase (DNAPL) concentrations of TCE over a wide aerial extent in relatively sandy soils with a shallow groundwater table. The investigation stemmed from concerns that ISCO remediation could have a variety of affects on the indigenous biological activity including reduced biodegradation rates and a long-term disruption of community structure with respect to the stimulation of TCE degraders. Phospholipid ester-linked fatty acid (PLFA) analyses of the aquifer material were used to assess structural and functional differences between microbial communities. The technique is independent of the bias inherent in classical culturing techniques and provides a more accurate estimation of in situ microbial populations. The data suggested that the ISCO treatment significantly increased the biomass of the test site sediments at 6 and 40 ft., and decreased the diversity in terms of the loss of Actinomycetes a the 6 ft. depth and oligotrophs at the 30 and 40 ft. depths. The 15 ft. samples had the lowest biomass across all sites and dates. The Recovery samples had slightly less biomass than the Oxidation samples, and there was not a great difference in their community structures.
URLs/Downloads:
LIPID ANALYSIS TO DETERMINE THE EFFECT OF A SOURCE REMEDIAL TECHNOLOGY IN MICROBIAL ECOLOGY (PDF, NA pp, 104 KB, about PDF)bioremedconf
