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DEVELOPMENTAL EXPRESSION OF ALDEHYDE DEHYDROGENASE IN RAT: A COMPARISON OF LIVER AND LUNG DEVELOPMENT
Citation:
YOON, M., M. C. MADDEN, AND H. A. BARTON. DEVELOPMENTAL EXPRESSION OF ALDEHYDE DEHYDROGENASE IN RAT: A COMPARISON OF LIVER AND LUNG DEVELOPMENT. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 89(2):386-398, (2006).
Impact/Purpose:
The objective of this study was to investigate the expression of Aldehyde dehydrogenases in liver and lung across ages
Description:
Metabolism is one of the major determinants for age-related susceptibility changes to chemicals. Aldehydes are highly reactive molecules present in the environment and can be produced during biotransformation of xenobiotics. Aldehyde dehydrogenases (ALDH) are important in aldehyde detoxification. Although the lung is a major target for aldehyde toxicity, development of ALDHs expression in lung has been poorly studied. The objective of this study was to investigate the expression of ALDH in liver and lung across ages (postnatal day1, 8, 22, and 60) in Wistar-Han rats. In adults, total lung ALDH activity was about 10 % of the hepatic level. The majority of hepatic ALDH activity was found in mitochondria while cytosol ALDH activity was the highest contributor in lung. Aldehyde oxidation ability in liver develops with age, but the ability remains constant in lung. The relative contribution of each isoform changes with age in a tissue specific manner, which is supported by the activity results in each subcellular fraction and the determination of ALDH1 and ALDH2 protein expression. Developmental expression of hepatic ALDH2 showed age-dependent increase while a different pattern was shown in lung. The relative contribution of hepatic ALDH2 was increased in adult about 4 fold higher than that of postnatal day1. These findings suggest that the liver and lung have differences in the composition of ALDH isoforms and their independent development with age determines the overall tissue-specific developmental profiles of ALDH expression. Collectively, the present study indicates that in the case of aldehyde exposure, the in vivo responses would be tissue and age-dependent.