You are here:
REAL TIME PCR ANALYSIS OF INDOOR MOLDS: PRINCIPLES, PROCEDURES AND APPLICATIONS
Citation:
HAUGLAND, R. A. REAL TIME PCR ANALYSIS OF INDOOR MOLDS: PRINCIPLES, PROCEDURES AND APPLICATIONS. Presented at Invited Seminar Presentation for Environmental Research Group, Atlanta, GA, April 29, 2005.
Impact/Purpose:
1. Develop and publish a standard method or guidance document for QPCR analysis of microorganisms in environmental samples (air and water filtrates and dust).a standard method or guidance document for QPCR analysis of microorganisms in environmental samples (air and water filtrates and dust). Publication will involve a consensus standards organization. 2. Use QPCR methods to monitor childhood exposures to mold as a part of field studies, in order to establish whether a relationship exists between molds encountered in indoor environments and asthma-related health problems.
Description:
This presentation will endeavor to present an overview of the real time polymerase chain reaction method developed for indoor mold detection and quantification by the EPA. It will begin with a brief discussion of the PCR technology that provides the basis for this method and how it is used to obtain quantitative results. The complete step by step method will then be illustrated followed by a discussion of the methodology used for designing specific PCR assays for different fungi. The presentation will conclude with two illustrations of how this method has been applied for the analyses of fungi in buildings and a summary of the perceived advantages of this method over conventional culture-based mold analyses.