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ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCAROMYCES CEREVISIAE
Citation:
Chen, C., T. G. Turi, D. Sanglard, AND J. C. Loper. ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCAROMYCES CEREVISIAE. doi:10.1016/0006-291, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. Elsevier Science, New York, NY, 146(3):1311-1317, (1987).
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Description:
We have isolated the gene for cytochrome P450 lanosterol 14-demethylase (14DM) from the yeast Candida tropicalis. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 3′ portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae.
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ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCAROMYCES CEREVISIAE