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GENETIC ENGINEERING OF ENHANCED MICROBIAL NITRIFICATION
Citation:
Carsiotos, M. AND S. Khanna. GENETIC ENGINEERING OF ENHANCED MICROBIAL NITRIFICATION. EPA/600/M-89/011 (NTIS 89-208334), 1989.
Impact/Purpose:
Information.
Description:
Experiments were conducted to introduce genetic information in the form of antibiotic or mercuric ion resistance genes into Nitrobacter hamburgensis strain X14. The resistance genes were either stable components of broad host range plasmids or transposable genes on methods for plasmid transformation as well as conjugation with various donor strains of Escherichia coli failed to achieve this goal. We also undertook the cloning of an origin of replication from strain X14; seven such experiments were unproductive. Both the leuB and thrB gene of strain X14 were successfully cloned by means of complementation of a leuB thrB auxotroph of E. Coli. The leuB gene of containing DNA was restriction-mapped and the 1.3 kilobase pair gene was subcloned into a vector suitable for use in DNA sequencing. To date, a tentative sequence comprising about 1300 bases has been obtained. There is an extensive similarity in three regions of the sequence with the amino acid sequence of the leuB gene product of Thermus thermophilus, Salmonella typhimurium, and Saccharomyces cerevisiae.