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PHARMACOKINETIC EVALUATION OF PERFLUOROOCTANOIC ACID IN THE MOUSE
Citation:
LAU, C. S., M. J. STRYNAR, A. B. LINDSTROM, R. HANSON, J. R. THIBODEAUX, AND H. A. BARTON. PHARMACOKINETIC EVALUATION OF PERFLUOROOCTANOIC ACID IN THE MOUSE . Presented at Society of Toxicology Meeting , New Orleans, LA, March 03 - 10, 2005.
Impact/Purpose:
The objective of this task is to develop state-of-the-art methods for measuring xenobiotic compounds, to include the isolation of the analyte from the appropriate matrix (extraction), preconcentration (typically sorbent-based), and analysis via GC/MS and/or LC/MS. Once established, these methods will be applied in small scale pilot studies or demonstration projects. Particular emphasis will be placed on methods which are readily transferable to other laboratories, including those within the Human Exposure and Atmospheric Sciences Division (HEASD), the National Exposure Research Laboratory (NERL), other EPA Laboratories, Program Offices, Regions, and academic institutions.
Specific objectives of this task include the following:
1) Development of GC/MS and LC/MS methods for the measurement of key xenobiotic compounds and their metabolites (to include the pyrethroid pesticides, perfluorinated organic compounds, and the BFRs) in relevant environmental and biological matrices.
2) Development of efficient low cost methods for the extraction and clean up of these compounds collected from relevant matrices.
3) Determination of xenobiotic compound and metabolite concentrations in samples derived from laboratory and field monitoring studies to help assess exposures and evaluate associated risks.
Description:
The poster is a collaboration between NHEERL and NERL. The NERL researchers developed methods for the analyses of Perfluorooctanoic Acids (PFOA) in various animal tissue isolates. The NHEERL researchers are developing an appropriate animal model to assess the distribution and deposition of PFOA in humans and to facilitate extrapolations of potential risks. For this collaboration NERL developed methods for the analyses of these compounds in mouse tissue and serum. The resulting data will be used to create new and more appropriate model of PFOA distribution and fate in animal systems.
Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy.