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ABRF-MARG RESEARCH STUDY: EVALUATION OF SMALL SAMPLE NUCLEIC ACID AMPLIFICATION TECHNOLOGIES FOR GENE EXPRESSION PROFILING
Citation:
LI, J., H. AUER, A. BROOKS, C. GRIFFIN, G. GRILLS, S. D. HESTER, G. KHITROV, K. KNUDTON, K. LILLEY, A. MASSIMI, J. P. TIESMAN, AND A. VIALE. ABRF-MARG RESEARCH STUDY: EVALUATION OF SMALL SAMPLE NUCLEIC ACID AMPLIFICATION TECHNOLOGIES FOR GENE EXPRESSION PROFILING. Presented at Association of Biomolecular Resource Facilities, Savannah, GA, February 05 - 09, 2005.
Description:
Microarrays have had a significant impact on many areas of biology. However, there are still many fertile research areas that would benefit from microarray analysis but are limited by the amount of biological material that can be obtained (e.g. samples obtained by small biopsy, fine needle aspiration, and Laser Capture Microdissection). In response to this demand, a number of companies have begun selling reagents capable of generating microarray targets from very small biological samples. To evaluate the relative merits of each of these technologies for the microarray community, the ABRF-MARG has developed a study to evaluate most commercial target amplification protocols and compare these to the standard target labeling procedure using the Affymetrix GeneChip platform. The results of this experiment will he presented. In addition to data quality (bias, noise, reproducibility, etc.) and comparison of the small sample protocols to the standard protocol, an evaluation of the technical merits of each procedure (ease of use, scalability, automatability, etc.) will be presented.