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INTEGRIN-MEDIATED CELL ATTACHMENT SHOWS TIME-DEPENDENT UPREGULATION OF GAP JUNCTION COMMUNICATION.
Citation:
Grindstaff, R D. AND C F. Blackman. INTEGRIN-MEDIATED CELL ATTACHMENT SHOWS TIME-DEPENDENT UPREGULATION OF GAP JUNCTION COMMUNICATION. Presented at American Society for Cell Biology, San Francisco, CA, 12/14-18/2002.
Description:
Integrin-mediated Cell Attachment Shows Time-Dependent Upregulation of Gap Junction
Communication
Rachel Grindstaff and Carl Blackman, National Health & Environmental Effects Research
Laboratory, Office of Research and Development, US EPA, Research Triangle Park, North Carolina 27711 USA
Integrin-mediated cell attachment to the extracellular matrix (ECM) has been shown to regulate cell proliferation, differentiation and development, migration, and possibly growth of certain carcinomas. Cell attachment to certain ECM constituents, fibronectin, laminin, and collagen IV, also has been shown to increase gap junction communication (GJC) and gap junction assembly in the cell membrane. We attached Clone 9 (normal rat liver, ATCC) cells to selected ECM substrates, measured cell-cell communication (scrape load/dye transfer), and stained for Connexin 43 (the primary connexin expressed in this cell line). ECM constituents, laminin (5uglcm2), collagen IV (5ug/cm2) or fibronectin (3uglcm2), were adsorbed onto tissue culture-treated plastic 35-mm dishes or 9mm2 glass coverslips for one hour at room temperature. Clone 9 cells were then plated at 2x10' 5 cells/dish, and allowed to attach and grow for 24 and 48 hours. After incubation, treatments were blinded. Functional cell to cell communication, as determined by transfer of Lucifer Yellow fluorescent dye (0.075% in PBS) between Clone 9 rat-liver epithelial cells, showed no difference between control and ECM substrates at each time point. Immunocytochemical staining of connexin 43 showed correlation with attachment time, not with matrix coating. For our rat liver epithelial cells, cell-to-cell communication, as well as increase in gap junction assembly, were found to be time dependent but not dependent upon attachment to specific ECM constituents.
Disclaimer: This abstract does not reflect EPA policy.