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ARRAY TECHNOLOGY AS A TOOL TO MONITOR ENDOCRINE DISRUPTION IN WILD FISH POPULATIONS
Citation:
Larkin, P., L C. Folmar, M J. Hemmer, P. Poston, H. S. Lee, AND N D. Denslow. ARRAY TECHNOLOGY AS A TOOL TO MONITOR ENDOCRINE DISRUPTION IN WILD FISH POPULATIONS. MARINE ENVIRONMENTAL RESEARCH 54(3-5):395-399, (2002).
Impact/Purpose:
In this study we describe the development of a sheepshead minnow estrogen responsive macroarray to investigate the feasibility of applying array technology in monitoring the environmental distribution of endocrine disrupting compounds that mimic estrogen.
Description:
A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technology as a monitoring tool for detecting the presence and distribution of estrogenic compounds in coastal habitats using sheepshead minnows as our model. cDNA clones that were isolated from differential display, including vitellogenin a and b, vitelline envelope protein (ZP2), and transferrin, among others, were spotted on the macroarray. The results of these experiments demonstrate a characteristic expression pattern of estrogen responsive genes in sheepshead minnows exposed to 17 b-estradiol (E2).