Citation:

FORTMANN, R. C. EVALUATION OF DISPOSABLE DIAPERS FOR QUANTATIVE MEASUREMENTS OF PESTICIDE METABOLITES AND CREATININE IN URINE SAMPLES. Presented at NCS Pilot Study Review, Research Triangle Park, NC, December 03, 2004.

Impact/Purpose:

The objective of this task is to identify, develop, and evaluate simple, cost effective monitoring methods that can be used to develop exposure classifications for the proposed Longitudinal Cohort Study. Methods once evaluated should be applicable to other epidemiological studies. This project will be conducted as four concurrent subprojects.

(1) demonstrating field performance and developing uptake rates of a semipermeable membrane devise (SMPD) to collect long-term integrated samples for semivolatile organics in air,

(2) developing simple rapid methods for analyzing the SMPDs,

(3) developing rapid field or near field methods for analyzing chemical metabolites in urine,

(4) review literature to identify available but not currently used techniques for long-term integrated exposure measurements.

Description:

This project consisted of a laboratory study to evaluate an extraction and analysis method for quantifying biomarkers of pesticide exposure and creatinine in urine samples collected with commercially-available disposable diapers. For large exposure studies, such as the National Children's Study (NCS), it is highly desirable to identify a low burden, low-cost method for collection of urine from infants and very young children. Disposable diapers that contain polyacrylate fibers or granules can be used to collect the urine samples if the pesticide metabolites can be quantitatively recovered from the diaper materials. The testing was performed in two phases. An initial set of tests was performed to evaluate the feasibility of the proposed solvent extraction and gas chromatography/mass spectrometry (GC/MS) analysis method. After demonstrating that the proposed method was feasible, a second set of tests was performed to document the performance of the method for metabolites of chlorpyrifos, diazinon, and synthetic pyrethroid pesticides. Laboratory testing has been completed. The testing showed that a single derivatization method could not be used for both the pyrethroid metabolites and the metabolites of chlorpyrifos and diazinon. The limit of detection for the method was 0.1 to 0.4 ng/mL for the ten metabolites that were tested. The efficiencies of extraction of the metabolites from the diapers ranged from 58% for cis-3-(2,2-dimethylvinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DMCA), a metabolite of synthetic pyrethroid pesticides to 102 % for 3,5,6-trichloro-2-pyridinol (TCPy), a metabolite of chlorpyrifos. The extraction efficiency was greater than 70% for eight of the 10 metabolites tested. The average extraction efficiency was 108% for creatinine. The results show that the method will be suitable for use in exposure measurement studies.

Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy.

Record Details: