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EXTRACTION AND DETECTION OF A NEW ARSINE SULFIDE CONTAINING ARSENOSUGAR IN MOLLUSCS BY IC-ICP-MS AND IC-ESI-MS/MS
Citation:
Fricke, M., P A. Creed, A N. Parks, J A. Shoemaker, C A. Schwegel, AND J T. Creed. EXTRACTION AND DETECTION OF A NEW ARSINE SULFIDE CONTAINING ARSENOSUGAR IN MOLLUSCS BY IC-ICP-MS AND IC-ESI-MS/MS. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 19(11):1454-1459, (2004).
Impact/Purpose:
To develop an arsenic speciation protocol for the analysis of dietary components and composite diets in order to provide a more accurate assessment of human exposure to arsenicals.
Description:
Using IC-ICP-MS and IC-ESI-MS/MS, an unknown arsenical compound in mollusks has been identified as a new arsine sulfide containing analog of a known arsenosugar and is referred to as As(498). This species has been observed in four separate shellfish species following a mild methanol/water extraction. As(498) is unstable, especially in acid, and converts to the arsine oxide containing arsenosugar As(482) over time.
Chromatographic retention of As(498) was observed on an anion exchanger ION-120 column but the species did not elute as a well defined peak from a PRP-X100. Mass spectrometric analysis of As(498) at pH 9.0 produced an [M-H]- species at a mass to charge of 497 in the negative-ion mode. A synthetic standard of As(498) was made by bubbling hydrogen sulfide into a stock solution of arsenosugar As(482). The retention time and ESI-MS/MS data were identical for the synthetic standard of As(498) and the unknown arsenical in s