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ACCUMULATION OF METHYLMERCURY OR POLYCHLORINATED BIPHENYLS IN IN VITRO MODELS OF RAT NEURONAL TISSUE.
Citation:
Meacham, C A., T. M. Freudenrich, W L. Anderson, L. Sui, T LyonsDarden, S Barone, M. E. Gilbert, W R. Mundy, AND T J. Shafer. ACCUMULATION OF METHYLMERCURY OR POLYCHLORINATED BIPHENYLS IN IN VITRO MODELS OF RAT NEURONAL TISSUE. TOXICOLOGY AND APPLIED PHARMACOLOGY. Elsevier Online, New York, NY, 205:177-187, (2005).
Description:
This manuscript reports data examining the accumulation of PCBs or methylmercury into the tissue of three commonly used in vitro neuronal models.
? The results demonstrate that these lipophilic compounds can accumulate to levels 5 to 100 fold higher than the surrounding solution, and that the amount of accumulation is dependent on the model system and the exposure conditions.
? The conclusion drawn from these studies is that the actual tissue content of toxicant is a better dose metric than the concentration in the surrounding solution. This is particularly true for comparisons to in vivo tissue levels.
? The ramifications if this study are: 1) it highlights the need to measure tissue concentrations in in vitro experiments; 2) it demonstrates the type of information needed to utilize in vitro data and make comparisons to in vivo tissue doses. This latter point is particularly important in constructing mode of action models, which often rely in part on in vitro data.
? These data highlight the need for similar studies of other classes of compounds.