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A SIMPLE METHOD FOR THE EXTRACTION AND QUANTIFICATION OF PHOTOPIGMENTS FROM SYMBIODINIUM SPP.
Citation:
ROGERS, JOHNE E. AND D. T. MARCOVICH. A SIMPLE METHOD FOR THE EXTRACTION AND QUANTIFICATION OF PHOTOPIGMENTS FROM SYMBIODINIUM SPP. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY. Elsevier Science Ltd, New York, NY, 353(2):191-197, (2007).
Impact/Purpose:
Procedure for the extraction and quantification of photopigments from Symbiodinium spp.
Description:
John E. Rogers and Dragoslav Marcovich. Submitted. Simple Method for the Extraction and Quantification of Photopigments from Symbiodinium spp.. Limnol. Oceanogr. Methods. 19 p. (ERL,GB 1192).
We have developed a simple, mild extraction procedure using methanol which, when coupled with HPLC-DAD analysis, quantifies the major photopigments found in Symbiodinium spp. Extracts were prepared by suspending wet cell pellets in methanol, soaking the cells for 2 hr in an ice bath, occasionally vortex mixing to resuspend the cells, and then adding 0.5 M ammonium acetate before storing the samples overnight at -20?C. Methanol extraction resulted in greater than 95 percent recovery of the four major photopigments, chlorophyll c, peridinin, diadinoxanthin, and chlorophyll a. Extraction by sonication in acetone:water (90:10) was less effective, 68% for chlorophyll a, 75% for diadinoxanthin, 49% for peridinin, and 50% for chlorophyll c.