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HEMOLYSIN, CHRYSOLYSIN FROM PENICILLIUM CHRYSOGENUM PROMOTES INFLAMMATORY RESPONSE
Citation:
Donohue, M J., Y. Chung, M L. Magnuson, M. W. Ward, M K. Selgrade, AND S J. Vesper. HEMOLYSIN, CHRYSOLYSIN FROM PENICILLIUM CHRYSOGENUM PROMOTES INFLAMMATORY RESPONSE. INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH. Urban & Fischer Verlag Jena, Jena, Germany, 208(4):279-285, (2005).
Impact/Purpose:
1. Evaluate the efficacy of QPCR technology for detecting low level microbiological contaminants in water supplies
2. Provide additional data on the range of pathogenic or potentially pathogenic species of fungi in community distribution system and hospital water samples.
Description:
Some strains of Penicillium chrysogenum produce a proteinaceous hemolysin, chrysolysin, when incubated on sheep's blood agar at 37 �C but not at 23 �C. Chrysolysin is an aggregating protein composed of approximately 2 kDa monomers, contains one cysteine amino acid, and has an isoelectric point of 4.85. Treatment of murine macrophage cell line RAW 264.7 with purified chrysolin caused statistically significant (T-test, p<0.05) increased productionj of macrophage inflammatory protein-2 (MIP-2) in a dose dependent manner after 6 h treatment. This suggests that chrysolysin might act to promote the host's inflammatory response after P. chrysogenum exposures.