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IMMUNOASSAY METHODS FOR MEASURING ATRAZINE AND 3,5,6-TRICHLORO-2-PYRIDINOL IN FOODS
Citation:
Van Emon, J M. AND J. C. Chuang. IMMUNOASSAY METHODS FOR MEASURING ATRAZINE AND 3,5,6-TRICHLORO-2-PYRIDINOL IN FOODS. Methods in Biotechnology. Humana Press Incorporated, Totowa, NJ, 19:231-238, (2006).
Impact/Purpose:
The overall objectives of the task include several components: (1) develop immunochemical methods for compounds difficult to analyze by conventional methodologies; (2) tailor immunochemical methods to support specific human exposure assessment studies; (3) team immunochemical sample preparations with instrumental analysis such as mass spectrometry for in-depth sample characterization; (4) provide methods to support NERL's human exposure and environmental monitoring efforts; (5) provide analytical methods that improve risk assessments by reducing the amount of uncertainity in environmental measurements; (6) provide multimedia analytical methods to support an integrated multimedia approach to assess and characterize risk to human health and the environment; (7) provide sponsorship of annual immunochemistry research meetings as a forum for stimulating interest and discussion on current or emerging bioanalytical methods; (8) develop and incorporate rapid, cost-effective laboratory and field portable immunochemical techniques such as enzyme-linked immunosorbent assay (ELISA) methods into monitoring studies and human exposure field surveys to delineate sub-populations of "highly exposed" individuals for detailed follow-up studies.
Specific method needs have been identified through consultations with client office personnel. This Task strives to fulfill those needs as appropriate. In particular, methods for pyrethroids, e.g., permethrin, cypermethrin, and deltamethrin are being developed and evaluated. Efficient sample preparations are under development for exposure samples using pressurized liquid extraction. Confirmation will be achieved using high performance liquid chromatography (HPLC). A rapid immunoassay approach for the analysis of 2,4-D in urine will be completed and a SOP and report written. Immunoaffinity chromatography sample preparations for the pyrethroids will be developed. Work will continue on the application of antibody replacements such as molecularly imprinted polymers. Additional candidate analytes will be identified for a tiered approach and to guide development of the next Task. Flexibility will be maintained to address methods and measurement issues as they arise during the task period which ends in FY06. The objective of the Task is to develop bioanalytical methods to support exposure monitoring studies during the task period.
Description:
This chapter describes the use of enzyme-linked immunosorbent assay (ELISA) methods for the analysis of two potential environmental contaminants in food sample media, atrazine and 3,5,6-trichloro-2-pyridinol (3,5,6-TCP). Two different immunoassay formats are employed: a magnetic-particle immunoassay testing kit and a 96-microwell plate immunoassay. Diluted, filtered, nonfat baby foods were analyzed directly by a commercial magnetic-particle immunoassay testing kit for the determination of atrazine. Fatty baby foods were extracted with water for the magnetic-particle immunoassay analysis of obtrusion. For 3,5,6-TCP analysis, the food samples from a duplicate diet exposure study were analyzed by a laboratory-based 96-micro well plate immunoassay. Acidic methanol (72% methanol, 26% water, and 2% acetic acid) was the solvent for the food samples.
The United States Environmental Protection agency (EPA) through its Office of Research and Development funded and collaborated in the research described here under contract No.68-D-99-011 to Battelle. It has been subjected to Agency review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by the EPA for use.