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MULTIPLE-ENDPOINT MUTAGENESIS WITH CHINESE HAMSTER OVARY (CHO) CELLS: EVALUATION WITH EIGHT CARCINOGENIC AND NON-CARCINOGENIC COMPOUNDS
Citation:
Hsie, A., J. Sebastian, S. Perdue, R. Schenley, AND M. Winters. MULTIPLE-ENDPOINT MUTAGENESIS WITH CHINESE HAMSTER OVARY (CHO) CELLS: EVALUATION WITH EIGHT CARCINOGENIC AND NON-CARCINOGENIC COMPOUNDS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-87/295 (NTIS PB88185137).
Description:
Using Chinese hamster ovary (CHO) cells in culture, the authors have defined an assay, CHO/HGPRT, to quantify mutagen-induced cytotoxicity and mutations at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus. This assay permits elucidation of the structure-activity relationship and analysis of relative mutagenic potency of various classes of chemical mutagens. By expanding the CHO/HGPRT assay to include chromosome aberration and sister-chromatid exchange (SCE), the authors can analyze the interrelationships of these four distinct biological effects and compare each endpoint for its predictive power of human-level effect. (Copyright (c) 1987 by Hemisphere Publishing Corporation.)