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Fate of pathologically bound oxygen resulting from inhalation of labeled ozone in rats
Citation:
Hatch, G., R. Slade, AND J. Mckee. Fate of pathologically bound oxygen resulting from inhalation of labeled ozone in rats. Environmental Health Insights. Libertas Academica Ltd, North Harbour, New Zealand, 7:43-58, (2013).
Impact/Purpose:
This is the first demonstration of ozone reaction products entering the circulation then the urine. Results have relevance to the mode of action of ozone, the identification of biomarkers for oxidative stress, and the interpretion of the adversity of formation of ozone adducts in the lung. The study exposed rats to oxygen-18 labeled ozone.
Description:
Inhaled ozone (03) reacts chemically with respiratory tissues where it forms adducts with most biomolecules. We quantified the plasma concentrations and urinary excretion of 18O in rats exposed to 1803 in order to gain insight into 0 injury and repair. Male Fischer 344 rats were exposed to l8O3 (2 ppm, 6 hr or 5 ppm, 2 hr) and urine was collected twice daily for 4 days. Increased 180 was detected in dried blood plasma at 7 hr post exposure and in dried urine for at least 4 days post exposure with higher excretion rates at night. Total 180 excreted was — 53% of the estimated amount of 18O3 retained by the rat during 18O3 exposure. Urinary 180 appeared to be of respiratory origin (not ingested), it was enriched in the >500 MW fraction, and it was stable to 250°C. Pre-exposure to unlabeled 03 a week earlier did not alter the excretion of 180 into urine following 18O3 exposure. We conclude that reaction products originating from 18O3 inhalation enter the circulation and are excreted into urine with only moderate recycling.
URLs/Downloads:
EPHD-13-069-ABSTRACT-FATAL.PDF (PDF, NA pp, 354.155 KB, about PDF)doi:10.4137/EHI.S12673
