EPA Science Inventory

Monitoring Intracellular Redox Changes in Ozone-exposed airway epithelial cells

Citation:

Gibbs-Flournoy, E., Steve Simmons, P. Bromberg, T. Dick, AND J. Samet. Monitoring Intracellular Redox Changes in Ozone-exposed airway epithelial cells. ENVIRONMENTAL HEALTH PERSPECTIVES. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, 121(3):312-7, (2013).

Description:

Background: The toxicity of many compounds involves oxidative injury to cells. Direct assessment of mechanistic events involved in xenobiotic-induced oxidative stress is not easily achievable. Development of genetically-encoded probes designed for monitoring intracellular redox status represents a methodological advance with potential applications in toxicological studies. Objective: Test the utility of redox sensors in monitoring intracellular redox status of toxicological studies involving xenobiotics. Methods: roGFP2, a redox reporter of the intracellular GSH/GSSG ratio, was used to monitor the redox potential of cultured BEAS 2B cells undergoing exposure to 0.15 – 1.0 ppm O3. Cells were imaged in real-time using a custom-built exposure system coupled to a confocal microscope. The roles of glutaredoxin 1 (Grx1), glutathione peroxidases (GPx), H2O2, and mitochondrial redox in transducing GSSG levels reported by roGFP in cells exposed to O3 were examined. Results: O3 exposure induced dose- and time- dependant losses in intracellular reducing potential. Grx1-linked roGFP2 was observed to enhance the kinetics of O3-induced loss of reducing potential while inhibition of endogenous Grx1 was shown to disrupt roGFP responses to oxidative insult. Selenite-induced GPx overexpression was observed to increase the rate of reducing potential losses. O3-induced H2O2 production and mitochondrial dysfunction did not appear to initiate the losses in reducing potential reported by cytosolic roGFP2. Conclusion: Exposure to O3 induces a profound loss of reducing potential in airway epithelial cells that is indicative of an oxidant-dependent impairment of redox homeostasis. These studies demonstrate the utility of using genetically-encoded reporters in making reliable assessments of cells undergoing exposure to xenobiotics with strong oxidizing properties.

Purpose/Objective:

This is a mechanistic study examining the oxidative effects of ozone on human lung cells.

URLs/Downloads:

EPHD-12-105-ABSTRACT.PDF   (PDF,NA pp, 42.756 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 03/28/2013
Completion Date: 03/28/2013
Record Last Revised: 06/20/2013
Record Created: 05/28/2013
Record Released: 05/28/2013
OMB Category: Other
Record ID: 256006

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB

ENVIRONMENTAL PUBLIC HEALTH DIVISION

CLINICAL RESEARCH BRANCH