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Analysis of PFOA in Dosed CD-1 Mice Part 2: Disposition of PFOA in Tissues and fluids from pregnant and lactating mice and their pups
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Citation:
FENTON, S. E., J. L. REINER, S. F. NAKAYAMA, A. DELINSKY, J. STANKO, E. P. HINES, S. WHITE, A. B. LINDSTROM, M. STRYNAR, AND S. E. PETROPOULOU. Analysis of PFOA in Dosed CD-1 Mice Part 2: Disposition of PFOA in Tissues and fluids from pregnant and lactating mice and their pups. REPRODUCTIVE TOXICOLOGY. Elsevier Science Ltd, New York, NY, 3(4):365-372, (2009).Description:
Previous studies in mice with multiple gestational exposures to perfluorooctanoic acid (PFOA) demonstrate numerous dose dependent growth and developmental effects which appeared to worsen if offspring exposed in utero nursed from PFOA-exposed dams. To evaluate the disposition of PFOA in the pregnant and lactating dam and her offspring, time-pregnant CD-1 mice received a single 0, 0.1, 1, or 5 mg PFOA/kg BW dose (N=25/dose group) by gavage on gestation day 17. Maternal and pup fluids and tissues were collected over time. Pups exhibited significantly higher serum PFOA concentrations than their respective dams, and their body burden increased after birth until at least 8 days old, regardless of dose. The distribution of milk:serum PFOA varied by dose and time, but was typically in excess of 0.20. These data suggest that milk is a substantial PFOA exposure route in mice and should be considered in risk assessment modeling designs for this compound.
Purpose/Objective:
This manuscript, submitted to Reproductive Toxicology's Special Issue on Perfluorinated Chemicals is a companion paper to one that was first authored and cleared through NERL, focusing strictly on method development for the data in this paper. This manuscript is the first to describe the temporal change in PFOA concentrations in the lactating mouse dam and the accumulation of exposure in her nursing pup during that important period of development. Milk appears to be a significant contributor to the pup body burden and should be considered in modeling exercises for risk assessment.
Record Details:
Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)Organization:
U.S. ENVIRONMENTAL PROTECTION AGENCYOFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
REPRODUCTIVE TOXICOLOGY DIVISION
DEVELOPMENTAL BIOLOGY BRANCH