EPA Science Inventory

IN VITRO CULTURING OF THE PREDATORY SOIL NEMATODE CLARKUS PAPILLATUS

Citation:

SALINAS, K. AND J. KOLTON. IN VITRO CULTURING OF THE PREDATORY SOIL NEMATODE CLARKUS PAPILLATUS. Nematology. Brill Academic Publishers, Boston, MA, 7(1):5-9, (2005).

Description:

Clarkus papillatus is a widely distributed predatory soil nematode and is of interest in the study of soil ecology, yet very little information exists on its in vitro culturing. In this investigation, an artificial environment was created to maintain C. papillatus for multi-generational studies. The mean number of C. papillatus increased significantly over time when starting the cultures with three adults per plate. Clarkus papillatus had a significantly higher median fecundity at 20, 25 and 28°C than at five other temperatures. Juvenile C. papillatus could not survive without adults in culture and, therefore, may require adult maceration of prey nematodes to survive in vitro.

Purpose/Objective:

To culture, in vitro, a widely distributed predatory soil nematode of interest in the study of soil ecology

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 02/01/2005
Completion Date: 02/01/2005
Record Last Revised: 03/27/2013
Record Created: 03/28/2006
Record Released: 03/28/2006
OMB Category: Other
Record ID: 151094

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB

GULF ECOLOGY DIVISION

BIOLOGICAL EFFECTS AND POPULATION RESPONSE BRANCH