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TIME AND CONCENTRATION DEPENDENT ACCUMULATION OF [3H]-DELTAMETHRIN IN XENOPUS LAEVIS OOCYTES.
Harrill, J., C A. Meacham, T J. Shafer, M F. Hughes, AND K M. Crofton. TIME AND CONCENTRATION DEPENDENT ACCUMULATION OF [3H]-DELTAMETHRIN IN XENOPUS LAEVIS OOCYTES. TOXICOLOGY LETTERS. Elsevier Online, New York, NY, 157:79-88, (2005).
Cell culture models are often used in mechanistic studies of toxicant action. However, one area of uncertainty is the extrapolation of dose from the in vitro model to the in vivo tissue. A common assumption of in vitro studies is that media concentration is a predictive marker of tissue concentration. This manuscript documents a series of in vitro experiments that characterize differences between media concentration and tissue dose for a pyrethroid insecticide. The data clearly demonstrate that tissue dose is dependent on media concentration, but is more influenced by incubation time. Depending on incubation time, media concentration either under-predicts or over-predicts tissue dose. The results of this work bring into question the ability to easily extrapolate from in vitro to in vivo. Accurate information on the concentration of a compound in cells should be determined experimentally.
To characterize differences between media concentration and tissue dose for a pyrethroid insecticide
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
NEUROBEHAVIORAL TOXICOLOGY BRANCH