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Exposure Research

Records 1 to 33 of 33
2015
Rhodes, E., L. Boczek, M. Ware, M. McKay, J. Hoelle, M. Schoen, AND E. Villegas. Determining pathogen and indicator levels in Class B municipal organic residuals used for land application. JOURNAL OF ENVIRONMENTAL QUALITY. American Society of Agronomy, MADISON, WI, 44(1):265-274, (2015).
2013
Brinkman, N., R. Francisco, T. Nichols, D. Robinson, F. Schaefer, P. Schaudies, AND E. Villegas. Detection of Multiple Waterborne Pathogens Using Microsequencing Arrays. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, 114(2):564-573, (2013).
Ware, M., S. Keely, AND E. Villegas. Development and evaluation of an off-the-slide genotyping technique for identifying Giardia cysts and Cryptosporidium oocysts directly from US EPA Method 1623 slides. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, 115(1):298-309, (2013).
Staggs, S., E. Beckman, S. Keely, R. Mackwan, M. Ware, A. Moyer, J. Ferretti, A. Sayed, L. Xiao, AND E. Villegas. The applicability of TaqMan-based quantitative real-time PCR assays for detecting and enumeratIng Cryptosporidium spp. oocysts in the environment. PLoS ONE . Public Library of Science, San Francisco, CA, 8(6):e66562, (2013).
2012
RHODES, E., L. F. VILLEGAS, N. J. Shaw, C. Miller, AND E. VILLEGAS. A Modified EPA Method 1623 that Uses Tangential Flow Hollow-Fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidum and Giardia spp. Journal of Visualized Experiments . JoVE, Somerville, MA, 65:E4177, (2012).
Raynal, M., E. VILLEGAS, AND K. L. Nelson. Enumeration of viable and non-viable larvated Ascaris eggs with quantitative PCR. JOURNAL OF WATER AND HEALTH. IWA Publishing, London, Uk, 10(4):594-604, (2012).
SEE, M. J., S. E. Staggs, J. P. Dubey, AND E. VILLEGAS. Evaluation of Four RNA Extraction Methods for Gene Expression Analyses of Cryptosporidium parvum and Toxoplasma gondii Oocys. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 89(3):185-192, (2012).
2011
RYU, H., T. TRAN, M. W. WARE, B. Iker, S. GRIFFIN, A. EGOROV, T. Edge, N. Newmann, E. N. VILLEGAS, AND J. W. SANTO-DOMINGO. Application of leftover sample material from waterborne protozoa monitoring for the molecular detection of <I>Bacteroidales</I> and fecal source tracking markers. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 86(3):337-343, (2011).
2010
RYU, H., B. Iker, J. W. SANTO-DOMINGO, M. W. WARE, E. VILLEGAS, AND T. TRAN. A New Sampling Strategy for the Detection of Fecal Bacteria Integrated with USEPA Method 1622/1623 . Presented at American Society for Microbiology 110th General Meeting, San Diego, CA, May 23 - 27, 2010.
WARE, M. W., S. J. AUGUSTINE, D. ERISMAN, M. J. See, L. J. WYMER, S. L. HAYES, J. P. Dubey, AND E. VILLEGAS. Determining UV Inactivation of Toxoplasma gondii Oocysts by Using Cell Culture and a Mouse Bioassay. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 76(15):5140-5147, (2010).
WARE, M. W. AND E. VILLEGAS. Improved Cryptosporidium parvum oocysts propagation using dexamethasone suppressed CF-1 mice . Veterinary Parasitology. Elsevier, Shannon, Ireland, 168(3-4):329-331, (2010).
Massie, G. N., M. W. WARE, E. VILLEGAS, AND M. W. Black. Uptake and transmission of Toxoplasma gondii oocysts by migratory filter-feeding fish. Veterinary Parasitology. Elsevier, Shannon, Ireland, 169(3-4):296-303, (2010).
2009
Brescia, C., S. Hunt, M. W. WARE, E. VARUGHESE, A. EGOROV, AND E. VILLEGAS. Cryptosporidium Propidium Monoazide-PCR, a Molecular Biology-Based Technique for Genotyping Viable Cryptosporidium Oocysts . APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 75(21):6856-6863, (2009).
Yang, W., H. LINDQUIST, V. Cama, F. W. SCHAEFER, E. VILLEGAS, R. Fayer, E. J. Lewis, Y. Feng, AND L. Xiao. Detection of Toxoplasma gondii oocuysts in water sample concentrates by real-time PCR. Journal of Environmental Microbiology. Blackwell Publishing, Malden, MA, 75(11):0099-2240, (2009).
VILLEGAS, E. Molecular-Based Detection Systems for Cryptosporidium Oocysts. Presented at 2009 USEPA STAR Grants Workshop on Innovative Approaches to Detecting Microorganisms and Cyanotoxins in Water, Philadelphia, PA, May 20 - 21, 2009.
Staggs, S. E., M. J. See, J. Dubey, AND E. VILLEGAS. Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient. Journal of Visualized Experiments . JoVE, Somerville, MA, (33):1-2, (2009).
VILLEGAS, E. Surveillance Systems for Waterborne Cryptosporidium: US EPA method 1523 and Beyond. Presented at 2009 USDA-CSREES National Water Conference, St. Louis, MO, February 08 - 12, 2009.
VILLEGAS, E. Surveillance Systems for Waterborne Protozoa Past, Present and Future. Presented at International Society of Exposure Sciences, Minneapolis, MN, November 01 - 05, 2009.
VILLEGAS, E. Surveillance Systems for Waterborne Protozoa: Beyond Method 1623. Presented at Greater Cincinnati Water Works Science Advisory Board Meeting, Cincinnati, OH, April 24, 2009.
VILLEGAS, E. Using Propidium Monoazide as an Enrichment Step to Remove Extraneous DMA and Non-Viable Organisms for Cryptosporidium Genotyping. Presented at Global Conference on Waterborne Microbial Contaminants, Singapore, SINGAPORE, October 05 - 08, 2009.
2008
WARE, M. W. AND E. VILLEGAS. Cryptosporidium Off-The-Slide Genotyping: Recovery of Oocysts Extracted from Slides Examined by Method 1623. Presented at Water Quality Technology Conference, Cincinnati, OH, November 16 - 20, 2008.
Wang, W., P. Chen, E. VILLEGAS, R. B. LANDY, C. KANETSKY, V. Cama, T. Dearen, C. Schultz, K. G. Orndorff, G. J. Prelewwicz, M. H. Brown, AND K. R. Young. Cryptosporidium Source Tracking in the Potomac River Watershed. Presented at 10th International Workshop on Opportunistic Protists, Boston, MA, May 28 - 31, 2008.
Yang, W., P. Chen, E. VILLEGAS, R. B. LANDY, C. KANETSKY, V. Cama, T. Dearen, C. L. Schultz, K. G. Orndorff, G. J. Prelewicz, M. H. Brown, K. YOUNG, AND L. Xiao. Cryptosporidium source tracking in the Potomac River watershed - MCEARD. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 74(21):6495-6504, (2008).
BRINKMAN, N., E. VILLEGAS, R. Francisco, F. W. SCHAEFER, T. NICHOLS, D. Roberts, AND P. Schaudies. Development of a Microarray Detection Method for Waterborne Pathogens - MCEARD/BARB. Presented at Joint EPA and DHS Conference on Real-World Applications and Solutions for Microbial Risk Assessment, Bethesda, MD, April 08 - 10, 2008.
AUGUSTINE, S. J., L. F. VILLEGAS, M. W. WARE, S. L. HAYES, M. J. See, J. P. Dubey, AND E. VILLEGAS. In-vitro Cell Culture and Real-time Reverse Transcriptase PCR-based Assays to Detect Infective Toxoplas gondii Oocysts. Presented at 2008 American Society for Microbiology Annual Meeting, Boston, MA, June 01, 2008.
Brescia, C., M. W. WARE, S. L. BARTELT-HUNT, A. EGOROV, AND E. VILLEGAS. Molecular Genotyping of Viable Cryptosporidium Oocysts. Presented at ASM Annual Meeting, Muncie, IN, March 28 - 29, 2008.
See, M. J., M. W. WARE, J. P. Dubey, AND E. VILLEGAS. RNA Extraction Methods for Real-Time PCR and Microarray Analyses of Cryptosporidium and Toxoplasma gondii Oocysts - 2nd Presentation. Presented at 10th International Workshop on Opportunistic Protists, Boxton, MA, May 28 - 31, 2008.
See, M. J., M. W. WARE, J. P. Dubey, AND E. VILLEGAS. RNA Extraction Methods for Reverse Transcriptase Real-Time PCR and Microarray Analysis of Cryptosporidium and Toxoplasma gondii Oocysts. Presented at ASM Annual Meeting, Muncie, IN, March 28 - 29, 2008.
2007
VILLEGAS, E. CRYPTOSPORIDIUM RESEARCH PROJECTS WITHIN THE BIOHAZARD ASSESSMENT RESEARCH BRANCH. Presented at Workshop on Cryptosporidium Research Associated with Monitoring and Analytical Efforts in the Mid-Atlantic, Philadelphia, PA, September 13, 2007.
YANG, W., P. CHEN, R. B. LANDY, C. KANETSKY, E. VILLEGAS, AND L. XIAO. CRYPTOSPORIDIUM SOURCE TRACKING TO ENHANCE SOURCE WATER PROTECTION IMPLEMENTATION IN THE POTOMAC RIVER WATERSHED: A REGIONAL APPLIED RESEARCH EFFORTS (RARE) PROJECT. Presented at U.S. EPA Region 5 Laboratory Technical Information Group Conference, Chicago, IL, April 30 - May 03, 2007.
VILLEGAS, E. AND N. BRINKMAN. MICROARRAY FOR DETECTION OF WATERBORNE PATHOGENS. Presented at 2007 Clean Water Partnership Summit, Cincinnati, OH, September 05 - 06, 2007.
2006
VILLEGAS, E., S. GLASSMEYER, M. W. WARE, S. L. HAYES, AND F. W. SCHAEFER. MATRIX ASSISTED LASER DESORPTION/IONIZATION TIME OF FLIGHT MASS SPECTROMETRY BASED ANALYSIS OF GIARDIA LAMBLIA. JOURNAL OF EUKARYOTIC MICROBIOLOGY. Allen Press, Inc., Lawrence, KS, 53(S1):S179-S-181, (2007).
VILLEGAS, E., S. GLASSMEYER, M. W. WARE, S. L. HAYES, AND F. W. SCHAEFER. MATRIX ASSISTED LASER DESORPTION/IONIZATION TIME OF FLIGHT MASS SPECTROMETRY BASED ANALYSIS OF GIARDIA LAMBLIA. Presented at IX International Workshop on Opportunistic Protists, Lisbon, PRINCIPLE, June 20 - 24, 2006.


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