Related Topics: Homeland Security Research

Homeland Security Research Products in the Science Inventory

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  • Keyword = ’homeland security’ AND ’sample processing and analysis' NOT (presentation OR poster).
Records 1 to 25 of 54

2023
Evaluation of two methods for detection of viable Bacillus anthracis simulant spores in maritime environmental samples
(JOURNAL) [Published : Jan 03, 2023]
Analytical methods exist to detect biothreat agents in environmental samples during a response to biological contamination incidents. However, the coastal zone facilities and assets of the United States Coast Guard (USCG), including response boats in diverse geographical areas an...
2022
Evaluation of Sample Processing Methods to Improve Detection of Bacillus anthracis in Difficult Sample Matrices
(JOURNAL) [Published : Oct 01, 2022]
Sub-product reports the evaluation and development of sampling extraction and processing procedures for difficult matrices samples. Such samples may contain high organic and environmental material, which will be challenging to process and analyze using the existing bio agent samp...
Protocol for Collection of Water Samples for Detection of Pathogens and Biothreat Agents
(PUBLISHED REPORT) [Published : Sep 30, 2022]
This document describes procedures for collecting and concentrating water samples in a field setting for waterborne pathogen and biothreat agent detection. The methods describe water sampling by concentrating large volumes of water in the field via dead-end ultrafiltration (DEUF)...
Protocol for Detection of Ricin Biotoxin in Environmental Samples During the Removal Phase of Response to a Contamination Incident
(PUBLISHED REPORT) [Published : Apr 30, 2022]
This is a protocol for detection of ricin biotoxin in environmental samples. It includes two sensitive and specific analytical methods including the ElectroChemiLuminescence-Based Immunoassay (ECL-IA) and the Enzyme-Linked Immunosorbent Assay (ELISA), also known as the Hand-Held ...
2021
Development of Rapid Viability-Reverse Transcriptase PCR (RV-RTPCR) Method for Detection of Infectious SARS-CoV-2 from Environmental Samples
(PUBLISHED REPORT) [Published : Sep 24, 2021]
This report describes the development of a Rapid Viability-Reverse Transcriptase PCR (RV-RTPCR) method for detection of infectious (live) virus (SARS-CoV-2) that causes COVID-19. The EPA-ORD’s Homeland Security Research Program (HSRP), in collaboration with the Department of Ener...
Evaluation of Analytical Methods for Detection of Bacillus anthracis Surrogate Spores: Compatibility with Real-World Maritime Environmental Samples Collected from the USCG Assets and Facilities
(PUBLISHED REPORT) [Published : Nov 01, 2021]
This product is a final report on the project on “Evaluation of Analytical Methods for Detection of Bacillus anthracis Surrogate Spores: Compatibility with Real-World Maritime Environmental Samples Collected from the U.S. Coast Guard (USCG) Assets and Facilities.” In this effort,...
Evaluation of a Modified Rapid Viability-Polymerase Chain Reaction Method for Bacillus atrophaeus Spores in Water Matrices
(JOURNAL) [Published : Sep 09, 2021]
A rapid method that provides information on the viability of organisms is needed to protect public health and ensure that remediation efforts following a release of a biological agent are effective. The rapid viability-polymerase chain reaction (RV-PCR) method combines broth cult...
2020
Optimization of a Rapid Viability Polymerase Chain Reaction (RV-PCR) Protocol for Detection of Francisella Tularensis in Water Samples
(PUBLISHED REPORT) [Published : Mar 18, 2020]
This is a final report for the project on Optimization of a Rapid Viability (RV) PCR Protocol for Detection of Francisella tularensis in water. During a tularemia bioterrorism incident or a natural outbreak, the water system can be contaminated. This pathogen survives for many da...
2019
Development of a Rapid Viability Polymerase Chain Reaction Method for Detection of Yersinia pestis in Water
(JOURNAL) [Published : May 13, 2019]
Due to the occurrence of natural plague outbreaks and its historical usage as a biological weapon, Yersinia pestis is considered one of the high-priority biological threat agents. It can remain viable in certain environments including water for more than 100 days. The Rapid Viabi...
Sample Processing Method for Ricin Analysis
(SUMMARY) [Published : Jan 30, 2019]
Technical Brief
The Use of Hollow Fiber Dialysis Filters Operated in Axial Flow Mode for Recovery of Microorganisms in Large Volume Water Samples with High Loadings of Particulate Matter
(JOURNAL) [Published : Apr 05, 2019]
The objective of this study was to evaluate axial flow hollow fiber ultrafiltration (HFUF) in the recovery of target organisms in large volumes of turbid water that would be similar to water encountered in the cleanup of a biologically contaminated area such as the B. anthracis c...
2018
Direct aqueous injection of the fluoroacetate anion in potable water for analysis by liquid chromatography tandem mass-spectrometry
(JOURNAL) [Published : Oct 29, 2018]
Journal Article describes the analysis of sodium fluoroacetate by liquid chromatography/tandem mass spectrometry. Four different drinking waters contaminated with the target analyte were evaluated.
Plastic Scintillation Fibers for Radiological Contamination Surveys
(PUBLISHED REPORT) [Published : Mar 28, 2018]
Report
Sampling, Laboratory and Data Considerations for Microbial Data Collected in the Field
(PUBLISHED REPORT) [Published : Jul 26, 2018]
Report: An informational companion to the MicroSAP tool which includes considerations for sampling, analysis, and data interpretation that users might include in a sampling and analysis plan being developed for a microbiological contamination incident.
Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017
(PUBLISHED REPORT) [Published : Jan 02, 2018]
Report (Methods)
2017
A Novel Broth Medium for Enhanced Growth of Francisella Tularensis
(JOURNAL) [Published : Feb 23, 2017]
Journal article
Analytical Protocol for VX Using Gas Chromatography/Mass Spectrometry (GC/MS)
(PUBLISHED REPORT) [Published : Sep 20, 2017]
Report
Joint USGS/USEPA Pathogens in Soils Geographic Information Systems Project
(DATA/SOFTWARE) [Published : Aug 02, 2017]
Online interactive maps
Processing Protocol for Soil Samples Potentially Contaminated with Bacillus anthracis Spores
(PUBLISHED REPORT) [Published : Mar 09, 2017]
Method Operating Procedures