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Immunochemical Determination of Dioxins in Sediment and Serum Samples

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Abstract: Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are considered highly toxic contaminants and the environmental and biological monitoring of these compounds is of great concern. Immunoassays may be used as screening methods to satisfy the growing demand for rapid and low cost analysis. In this work we describe the application of an immunoassay that uses 2,3,7-trichloro-8-methyldibenzo-p-dioxin (TMDD) as a surrogate standard for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to sediment and human serum samples. Sample extraction and preparation methods were developed with the aim to establish the simplest, cost-effective and efficient removal of the matrix interferences in the Enzyme-Linked Immunosorbent Assay (ELISA). The overall method for sediments is based on a hexane extraction; clean up by a multilayered silica gel column and an activated carbon column; an organic solvent exchange with DMSO-Triton X-100 and ELISA measurement. The gas chromatography - high resolution mass spectrometry (GC-HRMS) validation studies (n=13) revealed that the method is suitable for the TEQ (toxic equivalents) screening of dioxin in sediments with a method detection limit of about 100 pg g-1 dry sediment with a precision of 13-33%RSD. The analysis of a large number of samples originating from different sources would be required to establish more precisely the screening level, as well as the number of false positives and negatives of dioxin TEQ by the immunoassay for sediments. The immunoassay method for sediment analysis offers improvement in speed, sample throughput, and cost in comparison to GC-HRMS. Dioxins were determined in serum samples after a simple liquid-liquid extraction and solvent exchange into DMSO-Triton X-100 without further dilution. The current method (approximate method LOQ of 200 pg ml-1serum) is not sufficiently sensitive for the determination of dioxins in serum to measure acceptable exposure limit.

The U.S. Environmental Protection Agency through its Office of Research and Development (ORD) partially funded and collaborated in the research described here under contract No. GS-10F-00275K to Battelle. It has been subjected to the Agency's review and approved for publication. Mention of trade names and commercial products does not constitute endorsement or recommendation for use. This research is also funded by grants from the National Institute of Environmental Health Sciences 5 P42 ES04699 and NIEHS Center for Environmental Health Sciences P30 ES05707.
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Citation:Nichkova, M., E. Park, M. E. Koivunen, Kamita, S,G., S. J. Gee, J. C. Chuang, J. M. Van Emon, and B. D. Hammock. Immunochemical Determination of Dioxins in Sediment and Serum Samples. TALANTA 63(5):1213-1223, (2004).
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Exposure & Dose Research Branch
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Product Type: Journal
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Published: 08/01/2004
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Bullet Item Immunochemical Determination of Dioxins in Sediment and Serum Samples
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Related Entries:
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Bullet Item Field Analytical Methods: Advanced Field Monitoring Methods Development and Evaluation of New and Innovative Technologies That Support the Site Characterization and Monitoring Requirements of the Superfund Program.
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Last Updated on Monday, October 22, 2007
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