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Use of Pelleted Lettuce Seeds in Bioabailability Studies

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Abstract:Lettuce (Latuca sativa L., cv. Buttercrunch) is one of the most common and sensitive test organisms, among plants, used in toxicology and bioavailability studies. Much of the available lettuce seeds in commercial channels are pelleted to allow for precision machine planting. The use of pelletized seeds in plant studies would allow for a much greater diversity of seeds resources to be utilized. The following work compared the germination of pelleted, de-pelleted, and raw lettuce seeds to see if the pelleted seeds could be used in such studies. Pelleted and raw lettuce seeds were obtained from commercial sources. Pelleted seeds were de-pelleted in the lab by placing 100 seeds in a 50ml centrifuge tube, adding 30ml deionized water, and shaking gently for 3 min. The mix was filtered on qualitative filter paper in a Buchner funnel with vacuum for 3 min, and the filter paper was removed to air dry on the bench. Pelleted seeds contained approximately 10% seeds by weight, with the 90% pellet comprised of soluble and insoluble components. Pelleted, de-pelleted, and raw seeds were germinated in Petri dishes on two layers of germination blotter paper at 15oC with a 16h light/8h dark photoperiod (90 umol m-2 s-1 photosynthetic photon flux density (PPFD)). Total germination of the pelleted, de-pelleted, and raw seeds was identical (>90%) at 7 days. The de-pelleted and raw seeds had identical germination and growth time course plots. The pelleted seeds lagged behind in germination slightly up to 4 days, but were identical to the de-pelleted and raw seeds by 5 days. The work indicates that pelleted lettuce seeds could be used in plant toxicology or bioavailability studies provided such studies were longer than approximately 5-7 days.

This is an abstract of a proposed presentation and does not necessarily reflect the United States Environmental Protection Agency (EPA) policy. The actual presentation has not been peer reviewed by EPA. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.
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Citation:Cummings, K. T., R. Keys, K. Bradham, S. L. Harper, M. Medinavera, and A. Abushakra. Use of Pelleted Lettuce Seeds in Bioabailability Studies. Presented at The Southeastern Regional Meeting of the American Chemical Society, Research Triangle Park, NC, November 10-13, 2004.
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Methods Development & Application Branch
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Product Type: Abstrct/Oral
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Presented: 11/13/2004
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Related Entries:
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Bullet Item Toxic Elements: Development/Improvement of Traditional and Bioavailability Methods to Characterize Human and Ecological Exposure
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Last Updated on Monday, October 22, 2007
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